A1SDC7 (A1SDC7_NOCSJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

Noca_0267

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 EMBL ABL79812.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	248	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	285	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	381	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A1SDC7 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: ACA6FE27DC5973B5	FASTA	579	63,684
	10 20 30 40 50 60 MTHGPFDVWA PLPRRLRLAV AGETVAMRRG EGDWWTPAGP VPAGAELDYG YLVDDGEAPR 70 80 90 100 110 120 PDPRSRRQPG GVHQLSRTFD PASYPWTDQA WTGRQLAGSV VYELHVGTFT PEGTFDAALE 130 140 150 160 170 180 KLPHLREIGV DLVELMPVNA FNGTHNWGYD GVGWFAVHEG YGGPAGYQRF VDGCHAAGLG 190 200 210 220 230 240 VIQDVVYNHL GPSGNYLPEL GPYLKQGANT WGDFLNLDGP GSDEVRRYIL DNVRMWLADY 250 260 270 280 290 300 HVDGLRLDAV HALNDAGHQH LLEELAAEVA ALSAHQRRPL TLIAESDLND TRLVRPREAG 310 320 330 340 350 360 GYGLDAQWSD DFHHAVHVAL TGETAGYYAD FEPLSALAKV CERGFFHDGT WSSFRGRDHG 370 380 390 400 410 420 FPVDLRTMPT WRLVVCSQNH DQIGNRARGD RITEVLDDDQ LACAALLTLC GPFTPMLFMG 430 440 450 460 470 480 EEWAASTPFQ FFTSHPEPDL GTATAEGRIA EFERMGWDPA VVPDPQDPAT FERSKLDWAE 490 500 510 520 530 540 ATRGRHARML DVYRRLARLR REHEALTDPS FGSVRCTADE HTRVFTMRRG DLLVAVNFGD 550 560 570 AAATVETAAA TLLFQTGAGL TLDAGRLSLP PHAGALVGP

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL79812.1.
RefSeq	YP_921499.1. NC_008699.1.
3D structure databases
ProteinModelPortal	A1SDC7.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SDC7.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4596151.
GenomeReviews	Gene locus Noca_0267 in contig CP000509_GR.
KEGG	nca:Noca_0267.
PATRIC	22742519. VBINocSp122728_0529.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK2320911.
Enzyme and pathway databases
BioCyc	NSP35761:NOCA_0267-MONOMER.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1SDC7_NOCSJ

Accession

Primary (citable) accession number: A1SDC7

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)