A1SBC6 (PLSB_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Sama_3480

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049453
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A1SBC6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 247CFDC15679CC0A	FASTA	807	92,011
	10 20 30 40 50 60 MSAHESFWFK SLRWIQKQLV HTIVVPQDPF ADLNLDPSRP LVYVMKTESV SDIAALHEIT 70 80 90 100 110 120 GKLGLPSPYQ MLEIDGIKTP RVVCLEGRKP LFGKRDSNEP FLQTFQQLLA LHRQQQELDI 130 140 150 160 170 180 QLVPVSLYWG RTPGKEDDTM RAAVLEREDP TWLRKCLMIL FLGRHNFVQF SRAVSLRHMA 190 200 210 220 230 240 DEHGTDKRIA HKLARVARVH FRRQRKVMTG PVLPNRQAMF HALLKSDNLK KAIAEEASSK 250 260 270 280 290 300 KISEEKARET AIQYLDEIAA DYSDSLVRIA ERFLTWLWNK LYKGISIKGA EQIRQLHHDG 310 320 330 340 350 360 HEIVYVPCHR SHMDYLLLSY ILYYEGMVPP HIAAGINLNF WPAGPMFRRG GAFFIRRSFN 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFAKG YSVEYFTEGG RSRTGRLLAP KTGMLAMTLS SVIRGIERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKEKE SVWQVFGAIR KLGNFGRGYV NFGQPITLQN 490 500 510 520 530 540 FLTEKVPNWR EEVGEDPEQK PSWLTPVVNA LANRVMTRIN DAAAASSVTL SSMVLLASEQ 550 560 570 580 590 600 NALERNQLER QIDLYLSLLK SVPYTSYASV TEGCGKELVD RGIELNKLTE TKDDLGTIIS 610 620 630 640 650 660 IDDSLAISMT YYRNNIIHLF VIPSLIATVM VRHEEVSREE LQELVAEFYP LLKAELFMGV 670 680 690 700 710 720 TDLPAYVDAL VECFKSEGLI TGDNRLKLVD DRINQLLLLA GVVGETLKRY AIIFNLLGEQ 730 740 750 760 770 780 PRMERADLEH HSHRLASRLG AIHGVMAPEF YDKKLYALLS SKLKDLGYLS DKADGDKVRK 790 800 IRDHANGLLR SSVRQTIIET LNQEQDD

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000507 Genomic DNA. Translation: ABM01683.1.
RefSeq	YP_929352.1. NC_008700.1.
3D structure databases
ProteinModelPortal	A1SBC6.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SBC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4605727.
GenomeReviews	Gene locus Sama_3480 in contig CP000507_GR.
KEGG	saz:Sama_3480.
NMPDR	fig|326297.7.peg.3279.
PATRIC	23455893. VBISheAma74963_3588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHEAM

Accession

Primary (citable) accession number: A1SBC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program