A1SAR6 (KATG1_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 1
Short name=CP 1
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	Sama_3270

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354913
Sites
	Active site	94	1	Proton acceptor By similarity
	Metal binding	262	1	Iron (heme axial ligand) By similarity
	Site	90	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	93 ↔ 221		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-247) By similarity
	Cross-link	221 ↔ 247		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-93) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SAR6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 4F39F7F993749E34	FASTA	718	79,499
	10 20 30 40 50 60 MSNEGKCPVM HGGATSATQA DNHWWPKALN LDILHQHDSK TNPMAPGFNY REALKGLDVE 70 80 90 100 110 120 ALKQDLKALM TDSQAWWPAD WGHYGGLMIR MAWHSAGTYR IADGRGGGGH GAQRFAPLNS 130 140 150 160 170 180 WPDNGNLDKA RRLLWPIKQK YGNKVSWADL MILAGNMAYE SMGLKTFGFA FGREDIWHPE 190 200 210 220 230 240 KDTYWGAEKE WLAPSGGANS RYSGVRDLQN PLAAVMMGLI YVNPEGVDGN PDPLKTAQDM 250 260 270 280 290 300 RVTFARMAMN DEETVALTAG GHTVGKAHGN GNAANLGPEP EAAPLEEQGF GWMNHQSRGI 310 320 330 340 350 360 GRNTVTSGIE GAWTTHPTRW DNGYFHLLFS YDWALTKSPA GAWQWEPVNI REEDKPVDVE 370 380 390 400 410 420 DPNIRSNPMM TDADMALKMD PDYRRIAERF FHDPDYFADT FARAWFKLTH RDMGPKSRYF 430 440 450 460 470 480 GPDVPAEDLI WQDPVPKGNS QYDPEAVKAR IAGTGLGAAE LVATAWDSAR TYRNSDKRGG 490 500 510 520 530 540 ANGARIRLLP QKDWVANEPA RLARVLELLT PIASDMGVSI ADTLVLGGNL GVELAAKAAG 550 560 570 580 590 600 FNIKVPFIPG RGDASQAQTD IDSFEVLEPL ADGFRNWQKQ DFVVTPEEML LDRAQLMGLS 610 620 630 640 650 660 APEMTVLIGG MRAMAVNHGG TTHGVLTDNP GSLSNDFFVN LTDMSYRWKP VANNLYEICD 670 680 690 700 710 RASGQKKWTA TRVDLVFGSN SVLRAYAEYY AQDDNKETFV KDFVTAWCKV MNADRFDA

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000507 Genomic DNA. Translation: ABM01473.1.
RefSeq	YP_929142.1. NC_008700.1.
3D structure databases
ProteinModelPortal	A1SAR6.
SMR	A1SAR6. Positions 24-717.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SAR6.
Protein family/group databases
PeroxiBase	3656. SamCP02_SB2B.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4605517.
GenomeReviews	Gene locus Sama_3270 in contig CP000507_GR.
KEGG	saz:Sama_3270.
NMPDR	fig|326297.7.peg.3081.
PATRIC	23455453. VBISheAma74963_3369.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	A1SAR6.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_SHEAM

Accession

Primary (citable) accession number: A1SAR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program