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A1SAP5

- DAPF_SHEAM

UniProt

A1SAP5 - DAPF_SHEAM

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121SubstrateUniRule annotation
    Binding sitei45 – 451SubstrateUniRule annotation
    Binding sitei65 – 651SubstrateUniRule annotation
    Active sitei74 – 741Proton donor/acceptorUniRule annotation
    Binding sitei158 – 1581SubstrateUniRule annotation
    Sitei160 – 1601Important for catalytic activityUniRule annotation
    Binding sitei191 – 1911SubstrateUniRule annotation
    Sitei209 – 2091Important for catalytic activityUniRule annotation
    Active sitei218 – 2181Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciSAMA326297:GH0T-3369-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Sama_3249
    OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
    Taxonomic identifieri326297 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000009175: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275Diaminopimelate epimerasePRO_1000011959Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi74 ↔ 218

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi326297.Sama_3249.

    Structurei

    3D structure databases

    ProteinModelPortaliA1SAP5.
    SMRiA1SAP5. Positions 2-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni9 – 102Substrate bindingUniRule annotation
    Regioni74 – 763Substrate bindingUniRule annotation
    Regioni209 – 2102Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1SAP5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIHFTKMHGL GNDFMVVDGV TQNVFFSPEQ IRRLADRNFG IGFDQLLLVE    50
    PPYDPDLDFH YRIFNADGSE VEQCGNGARC FARFVRNKGL TQKHKIKVST 100
    SAGKMTLRLE RDGGVTVNMG VPILDPAQIP FKAKKFEKTY LLQSAQQTFL 150
    CGAVSMGNPH VVLEVDDITQ APVADVGAQL TRHERFPKGV NVGFMQVVAP 200
    DHIKLRVYER GAAETLACGS GACAAAVVGQ LQGKLGSRVR VDLPGGSLTI 250
    NWEGEGKPLW MTGPAQQVYD GQIQL 275
    Length:275
    Mass (Da):30,101
    Last modified:February 6, 2007 - v1
    Checksum:i4476A266BC880BA5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000507 Genomic DNA. Translation: ABM01452.1.
    RefSeqiWP_011761356.1. NC_008700.1.
    YP_929121.1. NC_008700.1.

    Genome annotation databases

    EnsemblBacteriaiABM01452; ABM01452; Sama_3249.
    GeneIDi4605496.
    KEGGisaz:Sama_3249.
    PATRICi23455401. VBISheAma74963_3347.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000507 Genomic DNA. Translation: ABM01452.1 .
    RefSeqi WP_011761356.1. NC_008700.1.
    YP_929121.1. NC_008700.1.

    3D structure databases

    ProteinModelPortali A1SAP5.
    SMRi A1SAP5. Positions 2-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 326297.Sama_3249.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM01452 ; ABM01452 ; Sama_3249 .
    GeneIDi 4605496.
    KEGGi saz:Sama_3249.
    PATRICi 23455401. VBISheAma74963_3347.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci SAMA326297:GH0T-3369-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1098 / SB2B.

    Entry informationi

    Entry nameiDAPF_SHEAM
    AccessioniPrimary (citable) accession number: A1SAP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3