A1SA53 (ALR_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	Sama_3057

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Alanine racemase HAMAP MF_01201		PRO_1000164608
Sites
	Active site	35	1	Proton acceptor; specific for D-alanine By similarity
	Active site	261	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	35	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SA53 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B3F87F93F2631A37	FASTA	364	39,409
	10 20 30 40 50 60 MKPFPRAEIS SRALKANLKR LRQIAPGSKV MAVVKANGYG HGLLNVAEVL TDAHSNADAD 70 80 90 100 110 120 GFGLARLEEA LEVRAGGVSA RLLLLEGFFR SEDLPLLVEH DIDTVVHHVS QLDMLESVSL 130 140 150 160 170 180 SKPVTVWLKI DSGMHRLGFH ASEFKDVYQR LQQNPNVAKP VHLMTHFSCA DEPQKDFTAT 190 200 210 220 230 240 QMAHFNALTQ GLPGDRTLAN SAGVLYWPQS QADWIRPGIA LYGVSPVAGD LGSNHGLEPA 250 260 270 280 290 300 MELVSQLIAV REHSAGESVG YGAYWTASRD TRLGVVAIGY GDGYPRNAPE GTPVLVNGRR 310 320 330 340 350 360 VPIVGRVSMD MLTVDLGPDA ADKVGDRALL WGKELPVEEV AERIGTIAYE LVTKLTPRVA VCLD

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000507 Genomic DNA. Translation: ABM01260.1.
RefSeq	YP_928929.1. NC_008700.1.
3D structure databases
ProteinModelPortal	A1SA53.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SA53.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4605304.
GenomeReviews	Gene locus Sama_3057 in contig CP000507_GR.
KEGG	saz:Sama_3057.
NMPDR	fig|326297.7.peg.2875.
PATRIC	23454999. VBISheAma74963_3146.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	EGTPVWV.
PhylomeDB	A1SA53.
ProtClustDB	PRK00053.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_SHEAM

Accession

Primary (citable) accession number: A1SA53

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program