Reviewed, UniProtKB/Swiss-Prot A1S9I4 (CYSI_SHEAM)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	Sama_2838

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta4. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000318562
Sites
	Metal binding	435	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	480	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	484	1	Iron (siroheme axial ligand) By similarity
	Metal binding	484	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1S9I4-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 99B82D9861DF90A7	FASTA	571	63,430
	10 20 30 40 50 60 MSQYPIKPEL QVQGQLSDNE RLKAESNHLR GTITTDLKDE ITGGFTGDNF MLVRFHGMYQ 70 80 90 100 110 120 QDDRDLRAER AEQKLEPLHN VMLRARMPGG IIKPEQWLAI DEFAREHTMY GSIRLTTRQT 130 140 150 160 170 180 FQFHGVFKPD IKPMHQMLNK IGIDSIATAG DVNRNVLCTS NPVESELHQQ AYEWAAKISE 190 200 210 220 230 240 HLLPKTRAYA EIWLGEEKVE TTETEPVLGD SYLPRKFKTT VVIPPHNDVD VHANDLNFVA 250 260 270 280 290 300 IAENGQLVGF NVLVGGGLAM THGDKSTYPR RAEDLGFIGL EHVLKVAEHV VTVQRDYGDR 310 320 330 340 350 360 VNRKNAKTKY TLDRIGVDAF KSEVEQRVGV AFGPSRPYEF THRGDRFGWV EGIDGKHHLT 370 380 390 400 410 420 LFIENGRLLD YPGKPLKTGV AEIAKVHKGD LRMTANQNLI IAGVAAEDKA QIEQLAREHG 430 440 450 460 470 480 LLDNVTTQRK NSMACVSLPT CPLAMAEAER YLPTLVTHVE GILSKHGIAD DHIILRVVGC 490 500 510 520 530 540 PNGCGRAMLA EAGLVGRGPG KYNLYLGGNT AGTRIPKLYL DNVAEAEILA ALDSLIGRWS 550 560 570 VERNPGECFG DFVIRVGVVA EVKVSKTDFH A

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000507 Genomic DNA. Translation: ABM01041.1.
RefSeq	YP_928710.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4605085.
GenomeReviews	Gene locus Sama_2838 in contig CP000507_GR.
KEGG	saz:Sama_2838.
NMPDR	fig|326297.7.peg.2659.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1S9I4. ITTTQWQ.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_SHEAM

Accession

Primary (citable) accession number: A1S9I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)