A1S8P4 (RLMG_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Sama_2548

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366504

Sequences

	Sequence		Length	Mass (Da)
	A1S8P4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 8E67B4A5AABEBCC3	FASTA	383	41,368
	10 20 30 40 50 60 MTTRFSAFLS GTPCELDLHR FPATSDPNLQ AWDAADEHLL KYLNESNTEL AAKFLVINDS 70 80 90 100 110 120 FGALTCALAT ARSDADIVFA ADGRTAHLGC KANLASNGLA SAKVDYQDCT NLGSFAKGRQ 130 140 150 160 170 180 ILMKLPKNLN FLTDTLSQLS QTLAAGDVIL MGAKAKHINQ SLLALIAKAV GPATASLTWK 190 200 210 220 230 240 KTRIITITAD GNPRPVSKPS VWDVPEHGLK VTNLSNVFAA SKLDIGARLM MANLPQGHFS 250 260 270 280 290 300 SVIDLGCGNG VLALKAAQTY PDARLYLVDE SAMAVESARQ NWALNALDEG RAEFIWDDCL 310 320 330 340 350 360 SHLPNEVQAD LVLCNPPFHQ GEAITDHIAW QMFNDAKRAL KPGGLLHIVG NRHLGYHIKL 370 380 KRLFGNCKTI ASNGKFVILQ AVK

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000507 Genomic DNA. Translation: ABM00751.1.
RefSeq	YP_928420.1. NC_008700.1.
3D structure databases
ProteinModelPortal	A1S8P4.
ModBase	Search...
Protein-protein interaction databases
STRING	A1S8P4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4604795.
GenomeReviews	Gene locus Sama_2548 in contig CP000507_GR.
KEGG	saz:Sama_2548.
NMPDR	fig|326297.7.peg.2388.
PATRIC	23453907. VBISheAma74963_2621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
PhylomeDB	A1S8P4.
ProtClustDB	CLSK906089.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHEAM

Accession

Primary (citable) accession number: A1S8P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program