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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei239ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAMA326297:G1G7Q-2331-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Sama_2207
OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Taxonomic identifieri326297 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000009175 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019551 – 469Glutamate--tRNA ligaseAdd BLAST469

Proteomic databases

PRIDEiA1S7Q6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi326297.Sama_2207

Structurei

3D structure databases

ProteinModelPortaliA1S7Q6
SMRiA1S7Q6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi236 – 240"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A1S7Q6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKTRFAPS PTGFLHVGGA RTALYSWLHA RANQGEFVLR IEDTDLERST
60 70 80 90 100
QEAVDAILEG MEWLNLNWDE GPYYQTKRFD RYNEIIDQML KAGTAYKCYC
110 120 130 140 150
SKERLEAVRE EQAAKGERQK YDGCCRGAAP RAEGEPHVVR FLNPQGGSVV
160 170 180 190 200
FDDHVRGRIE IANEELDDLI IRRTDGSPTY NFCVVVDDWD MGITHVVRGE
210 220 230 240 250
DHINNTPRQI NILKALGAPI PEYAHVSMIL GDDGAKLSKR HGAVSVMQYR
260 270 280 290 300
DDGYLPEALL NYLVRLGWSH GDQEIFSMDE LVEFFRLDDI NKAASAFNSE
310 320 330 340 350
KLLWLNQHYI KALDPEYVAK HLEWHMKDQG IDTSNGPALA DVVTALSERA
360 370 380 390 400
KTLKELAASS RYFYEDFEEF DADQAKKHLR GVALEPLKLV QQKLAALTEW
410 420 430 440 450
TREAIHQAIE ETATELEVGM GKVGMPLRVA VTGAGQSPGL DITLELIGKA
460
RSEQRISKAV DFVADRINS
Length:469
Mass (Da):52,814
Last modified:February 6, 2007 - v1
Checksum:iEEAD02603E55A65E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000507 Genomic DNA Translation: ABM00413.1
RefSeqiWP_011760320.1, NC_008700.1

Genome annotation databases

EnsemblBacteriaiABM00413; ABM00413; Sama_2207
KEGGisaz:Sama_2207

Similar proteinsi

Entry informationi

Entry nameiSYE_SHEAM
AccessioniPrimary (citable) accession number: A1S7Q6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health