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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Binding sitei170S-adenosyl-L-methionineUniRule annotation1
Binding sitei188S-adenosyl-L-methionineUniRule annotation1
Active sitei241NucleophileUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:Sama_2039
OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Taxonomic identifieri326297 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000009175 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002850091 – 467Ribosomal RNA small subunit methyltransferase FAdd BLAST467

Interactioni

Protein-protein interaction databases

STRINGi326297.Sama_2039.

Structurei

3D structure databases

ProteinModelPortaliA1S788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 125S-adenosyl-L-methionine bindingUniRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiPOG091H02E2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiIPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1S788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQLNQKFIN SIAQEMPPHL SMDDFVHYCG LPLRLSIRVN TLKITSDALR
60 70 80 90 100
AILEPRGWQF EPVPWCEDGF WVTVPDDCQP GNLTEHYQGL FYIQEASSMM
110 120 130 140 150
PPCALFMDKE PRQLLLDVAS APGSKTTQLA ALMHNQGLII ANEYSASRTK
160 170 180 190 200
ALHANLQRMG VANVAITQFD GRVFGAHLYE TLDAIQLDAP CSGEGTVRKD
210 220 230 240 250
PLSLKNWCPD EIEAIAELQR DLIDSAFQAL KPGGVLVYST CTLNRRENED
260 270 280 290 300
VCHFLKERYG DAVVFESLND LFKGADKALT EEGFLHIWPQ IYDSEGFFVA
310 320 330 340 350
RIRKTASVAR NTDDPRFVSK FPFVAANRKE LSALEEAMLA LGVSLPEDAV
360 370 380 390 400
IVTRDGEFWL MPAPLDGLLT KMRFQRIGIR LAETQKHGIK VRHEAVMALP
410 420 430 440 450
CNQMLSIEPE AAKQYLMGRD IALDNAGKAQ GEKILSLHGA PLGIAKHLGN
460
KLKNSLPRDL CRDNVQN
Length:467
Mass (Da):52,100
Last modified:February 6, 2007 - v1
Checksum:i52CDFBF24F60E06D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000507 Genomic DNA. Translation: ABM00245.1.
RefSeqiWP_011760152.1. NC_008700.1.

Genome annotation databases

EnsemblBacteriaiABM00245; ABM00245; Sama_2039.
KEGGisaz:Sama_2039.
PATRICi23452831. VBISheAma74963_2104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000507 Genomic DNA. Translation: ABM00245.1.
RefSeqiWP_011760152.1. NC_008700.1.

3D structure databases

ProteinModelPortaliA1S788.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326297.Sama_2039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM00245; ABM00245; Sama_2039.
KEGGisaz:Sama_2039.
PATRICi23452831. VBISheAma74963_2104.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiPOG091H02E2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiIPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMF_SHEAM
AccessioniPrimary (citable) accession number: A1S788
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.