Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1S6V2 (DDL_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Sama_1903
OrganismShewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]
Taxonomic identifier326297 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074783

Regions

Domain124 – 330207ATP-grasp
Nucleotide binding154 – 20956ATP By similarity

Sites

Metal binding2841Magnesium or manganese 1 By similarity
Metal binding2971Magnesium or manganese 1 By similarity
Metal binding2971Magnesium or manganese 2 By similarity
Metal binding2991Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1S6V2 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: B9006C40E16E482E

FASTA33637,815
        10         20         30         40         50         60 
MNKYNLVLLC GGGGSEHDIS LLSAKYFESC LATLPRFNTL WLELTAEGQF RTREGEIVEL 

        70         80         90        100        110        120 
TNRREIRFAD EAKAPWSVDY VIPCIHGYPG ETGDIQSWFN LIRLPYFGCG SEASSNCFNK 

       130        140        150        160        170        180 
ITAKMWFSAL GIPNTPYLYL DEPSNDAIAR VEAAFDEWGS VFIKAASQGS SVGCFPAHRR 

       190        200        210        220        230        240 
EDIPGLVRKA FEYAPFVVVE KTIKARELEV AVYDYQGEIV ATRPGEIVCA ANTFYSFEEK 

       250        260        270        280        290        300 
YDTKSQAVTH VEAPDVDEET VAAIRDYALR AFKGMKLRHL SRIDFFLTED NQILLNEINT 

       310        320        330 
FPGLTQISMF PKMLAHHGHD FATFLEQAIL AELEGR 

« Hide

References

[1]"Complete sequence of Shewanella amazonensis SB2B."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000507 Genomic DNA. Translation: ABM00109.1.
RefSeqYP_927778.1. NC_008700.1.

3D structure databases

ProteinModelPortalA1S6V2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326297.Sama_1903.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM00109; ABM00109; Sama_1903.
GeneID4604153.
KEGGsaz:Sama_1903.
PATRIC23452547. VBISheAma74963_1962.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycSAMA326297:GH0T-1980-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SHEAM
AccessionPrimary (citable) accession number: A1S6V2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways