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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 65ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAMA326297:G1G7Q-1407-MONOMER
UniPathwayiUPA00359; UER00482

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:Sama_1338
OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Taxonomic identifieri326297 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000009175 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002912411 – 349Tetraacyldisaccharide 4'-kinaseAdd BLAST349

Proteomic databases

PRIDEiA1S589

Interactioni

Protein-protein interaction databases

STRINGi326297.Sama_1338

Structurei

3D structure databases

SMRiA1S589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD Bacteria
COG1663 LUCA
HOGENOMiHOG000004953
KOiK00912
OMAiMDDGFQN
OrthoDBiPOG091H000L

Family and domain databases

HAMAPiMF_00409 LpxK, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR003758 Tetraacyldisaccharide_4-kinase
PANTHERiPTHR42724 PTHR42724, 1 hit
PfamiView protein in Pfam
PF02606 LpxK, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00682 lpxK, 1 hit

Sequencei

Sequence statusi: Complete.

A1S589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSFVHKIWY QGHPAAWLLL PLSLLFWLVS SLRRAAFRLG LKRSERLPVP
60 70 80 90 100
VVVVGNITAG GSGKTPTVLY LIELLRREGW RPGVISRGYG ASFDGELDVV
110 120 130 140 150
AGMSPAQVGD EPAMIAMRTG IPMVVGRNRI KAAHKLLQCH DVNVILCDDG
160 170 180 190 200
LQHYALERDV EILVIDGERR FGNGWLLPAG PLREGAWRKN SVNFVLCNGA
210 220 230 240 250
SAEADEYAMT LEPTGLVAVA AIHGKSQAKD NSHNAQDNAT PRPGDTVNAM
260 270 280 290 300
AGIGNPQRFF STLMAQGFVL EKAHEFADHM AFSETDIATI DDGRPLLMTE
310 320 330 340
KDAVKCREFA KQHWWYLAVD ANLPPTFSTR LLDALNRAAT AKQGNTHGL
Length:349
Mass (Da):38,227
Last modified:February 6, 2007 - v1
Checksum:iB232867C020C8490
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000507 Genomic DNA Translation: ABL99545.1
RefSeqiWP_011759453.1, NC_008700.1

Genome annotation databases

EnsemblBacteriaiABL99545; ABL99545; Sama_1338
KEGGisaz:Sama_1338

Similar proteinsi

Entry informationi

Entry nameiLPXK_SHEAM
AccessioniPrimary (citable) accession number: A1S589
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: February 6, 2007
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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