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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69L-glutamineUniRule annotation1
Binding sitei214L-glutamineUniRule annotation1
Binding sitei233ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 39ATPUniRule annotation3
Nucleotide bindingi43 – 49ATPUniRule annotation7
Nucleotide bindingi266 – 267ATPUniRule annotation2
Nucleotide bindingi274 – 276ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation)
Alternative name(s):
Glutaminyl-tRNA synthetaseUniRule annotation
Short name:
GlnRSUniRule annotation
Gene namesi
Name:glnSUniRule annotation
Ordered Locus Names:Sama_1216Imported
OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)Imported
Taxonomic identifieri326297 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000009175 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi326297.Sama_1216.

Structurei

3D structure databases

ProteinModelPortaliA1S4W7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 342tRNA-synt_1cInterPro annotationAdd BLAST313
Domaini345 – 534tRNA-synt_1c_CInterPro annotationAdd BLAST190

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi36 – 46"HIGH" regionUniRule annotationAdd BLAST11
Motifi273 – 277"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CX6. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000259232.
KOiK01886.
OMAiVTHSICT.
OrthoDBiPOG091H00G9.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

A1S4W7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHVDNEVRP SNFIRNIIDE DLASGKHTSV HTRFPPEPNG YLHIGHAKSI
60 70 80 90 100
CLNFGIAEDY KGQCNLRFDD TNPEKEDIDY VNSIQEDVKW LGFQWDGEVR
110 120 130 140 150
YSSNYFDELY GYAVELINKG LAYVCFLNAE ETREYRGTLK EPGRNSPYRD
160 170 180 190 200
TSPEENLALF EKMRDGGFKE GECVLRAKID MASPFMCMRD PVIYRVKFAH
210 220 230 240 250
HHQTGDKWCI YPMYDFTHCI SDALENITHS LCTLEFQDNR RLYDWVLDNL
260 270 280 290 300
NDFQAPNRTR QYEFSRLNLE YTLMSKRKLN DLVVRGLVSG WDDPRMPTIA
310 320 330 340 350
GLRRRGYTPA SIREFCQRIG VTKQDNLVEV GMLEACIRED LNENAPRAMA
360 370 380 390 400
VIRPIKVIIE NFTAPELEYV KAPVHPNLEE MGERELAFGR EIYIDAADFR
410 420 430 440 450
EEANKQYKRL VLGKEVRLRN AYVIRAERCE KDAEGNVTTV YCTYDPETLG
460 470 480 490 500
QNPSDGRKVK GVIHWVEATT ALPAEFRLYD RLFIDANPAA AETVDEVLNP
510 520 530 540 550
ASLEVVHGFA EAPLANAKAS QAYQFEREGY FCADSKDSAP GKLVFNLTVA

LRESF
Length:555
Mass (Da):63,731
Last modified:February 6, 2007 - v1
Checksum:i6CD13A8FDBEBA115
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000507 Genomic DNA. Translation: ABL99423.1.
RefSeqiWP_011759332.1. NC_008700.1.

Genome annotation databases

EnsemblBacteriaiABL99423; ABL99423; Sama_1216.
KEGGisaz:Sama_1216.

Similar proteinsi

Entry informationi

Entry nameiA1S4W7_SHEAM
AccessioniPrimary (citable) accession number: A1S4W7
Entry historyiIntegrated into UniProtKB/TrEMBL: February 6, 2007
Last sequence update: February 6, 2007
Last modified: September 27, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported