A1S4U5 (GH109_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycosyl hydrolase family 109 protein
EC=3.2.1.-

Gene names

Ordered Locus Names:

Sama_1194

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella amazonensis

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glycosidase By similarity.
Cofactor	Binds 1 NAD+ per subunit. The NAD cannot dissociate By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the Gfo/Idh/MocA family. Glycosyl hydrolase 109 subfamily.

Ontologies

Keywords
Domain	Signal
Ligand	NAD
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	oxidation-reduction process Inferred from electronic annotation. Source: GOC
Molecular_function	hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Tat-type signal Potential
	Chain	34 – 455	422	Glycosyl hydrolase family 109 protein		PRO_5000205145
Regions
	Nucleotide binding	62 – 63	2	NAD By similarity
	Nucleotide binding	133 – 136	4	NAD By similarity
	Nucleotide binding	153 – 154	2	NAD By similarity
	Region	242 – 245	4	Substrate binding By similarity
Sites
	Binding site	84	1	NAD By similarity
	Binding site	112	1	NAD; via carbonyl oxygen By similarity
	Binding site	182	1	NAD By similarity
	Binding site	211	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity
	Binding site	242	1	NAD By similarity
	Binding site	324	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1S4U5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 420B511BBCB00967	FASTA	455	50,618
	10 20 30 40 50 60 MAGIDRRGFL KASMASVAAA ALAGCASQQG TSATPKAAGK SVMGLVVPKM AEVRVGLIGV 70 80 90 100 110 120 GERGVGFIHH FNNIEGARIT AICDTDPLVI SRAQKIMADY GRSQPAYYSK GQQAYLDLVA 130 140 150 160 170 180 REDVDIVVIA TPWALHHPMA KAAMLAGKHA FVEVPMGMTI EELWDLVDTA ELTQRNCMMM 190 200 210 220 230 240 ENVCYGRDEL MVLNMVRQGL FGELLHGEAA YIHELRWQMK ELDRKTGSWR TGYHAQINGN 250 260 270 280 290 300 LYPTHGLGPV AQYMNINRGD RFDYLSSMSS PALGRAAYAQ REFPKDHQRN QLNYICGDMN 310 320 330 340 350 360 TSLIKTVKGR TIMVQHDTTT PRPYSRHNLI QGTNGVFAGF PNRIALENHG RGSFHEWDQD 370 380 390 400 410 420 MEHWYGKYDH PLWTRMGREA EQNGGHGGMD FLMCWRMIYC LRNGEPLDQD VYDGAAWSAV 430 440 450 QPLSAASVAD RGNSRDFPDF TRGVWQSATP LGIVE

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000507 Genomic DNA. Translation: ABL99401.1.
RefSeq	YP_927071.1. NC_008700.1.
3D structure databases
ProteinModelPortal	A1S4U5.
ModBase	Search...
Protein-protein interaction databases
STRING	326297.Sama_1194.
Protein family/group databases
CAZy	GH109. Glycoside Hydrolase Family 109.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABL99401; ABL99401; Sama_1194.
GeneID	4603446.
KEGG	saz:Sama_1194.
PATRIC	23451027. VBISheAma74963_1234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0673.
HOGENOM	HOG000252553.
OMA	PVAQYMN.
ProtClustDB	CLSK901939.
Enzyme and pathway databases
BioCyc	SAMA326297:GH0T-1324-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR016040. NAD(P)-bd_dom. IPR000683. Oxidoreductase_N. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF01408. GFO_IDH_MocA. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GH109_SHEAM

Accession

Primary (citable) accession number: A1S4U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program