Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1S4U5 (GH109_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycosyl hydrolase family 109 protein

EC=3.2.1.-
Gene names
Ordered Locus Names:Sama_1194
OrganismShewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]
Taxonomic identifier326297 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length455 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycosidase By similarity.

Cofactor

Binds 1 NAD+ per subunit. The NAD cannot dissociate By similarity.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Sequence similarities

Belongs to the Gfo/Idh/MocA family. Glycosyl hydrolase 109 subfamily.

Ontologies

Keywords
   DomainSignal
   LigandNAD
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processoxidation-reduction process

Inferred from electronic annotation. Source: GOC

   Molecular_functionhydrolase activity, acting on glycosyl bonds

Inferred from electronic annotation. Source: UniProtKB-KW

oxidoreductase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333Tat-type signal Potential
Chain34 – 455422Glycosyl hydrolase family 109 protein
PRO_5000205145

Regions

Nucleotide binding62 – 632NAD By similarity
Nucleotide binding133 – 1364NAD By similarity
Nucleotide binding153 – 1542NAD By similarity
Region242 – 2454Substrate binding By similarity

Sites

Binding site841NAD By similarity
Binding site1121NAD; via carbonyl oxygen By similarity
Binding site1821NAD By similarity
Binding site2111Substrate By similarity
Binding site2301Substrate By similarity
Binding site2421NAD By similarity
Binding site3241Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1S4U5 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 420B511BBCB00967

FASTA45550,618
        10         20         30         40         50         60 
MAGIDRRGFL KASMASVAAA ALAGCASQQG TSATPKAAGK SVMGLVVPKM AEVRVGLIGV 

        70         80         90        100        110        120 
GERGVGFIHH FNNIEGARIT AICDTDPLVI SRAQKIMADY GRSQPAYYSK GQQAYLDLVA 

       130        140        150        160        170        180 
REDVDIVVIA TPWALHHPMA KAAMLAGKHA FVEVPMGMTI EELWDLVDTA ELTQRNCMMM 

       190        200        210        220        230        240 
ENVCYGRDEL MVLNMVRQGL FGELLHGEAA YIHELRWQMK ELDRKTGSWR TGYHAQINGN 

       250        260        270        280        290        300 
LYPTHGLGPV AQYMNINRGD RFDYLSSMSS PALGRAAYAQ REFPKDHQRN QLNYICGDMN 

       310        320        330        340        350        360 
TSLIKTVKGR TIMVQHDTTT PRPYSRHNLI QGTNGVFAGF PNRIALENHG RGSFHEWDQD 

       370        380        390        400        410        420 
MEHWYGKYDH PLWTRMGREA EQNGGHGGMD FLMCWRMIYC LRNGEPLDQD VYDGAAWSAV 

       430        440        450 
QPLSAASVAD RGNSRDFPDF TRGVWQSATP LGIVE 

« Hide

References

[1]"Complete sequence of Shewanella amazonensis SB2B."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000507 Genomic DNA. Translation: ABL99401.1.
RefSeqYP_927071.1. NC_008700.1.

3D structure databases

ProteinModelPortalA1S4U5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326297.Sama_1194.

Protein family/group databases

CAZyGH109. Glycoside Hydrolase Family 109.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL99401; ABL99401; Sama_1194.
GeneID4603446.
KEGGsaz:Sama_1194.
PATRIC23451027. VBISheAma74963_1234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0673.
HOGENOMHOG000252553.
OMACYGRDEL.
OrthoDBEOG61P6MK.
ProtClustDBCLSK901939.

Enzyme and pathway databases

BioCycSAMA326297:GH0T-1238-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF01408. GFO_IDH_MocA. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
TIGRFAMsTIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGH109_SHEAM
AccessionPrimary (citable) accession number: A1S4U5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 6, 2007
Last modified: November 13, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries