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A1S4D9 (ISPD_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT
Gene names
Name:ispD
Ordered Locus Names:Sama_1038
OrganismShewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]
Taxonomic identifier326297 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108

Sequence similarities

Belongs to the IspD family.

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2382382-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
PRO_1000022947

Sites

Site231Transition state stabilizer By similarity
Site301Transition state stabilizer By similarity
Site1611Positions MEP for the nucleophilic attack By similarity
Site2171Positions MEP for the nucleophilic attack By similarity

Sequences

Sequence LengthMass (Da)Tools
A1S4D9 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: CEDC4178550BDDDC

FASTA23825,427
        10         20         30         40         50         60 
MNFQSSSSGS IVAIVPAAGI GSRMGATIPK QYLPLLDKPI LAHTLQRLLS HPAIDKVIVA 

        70         80         90        100        110        120 
VSAEDSWFDS LAEARDPKLT RVLGGKERAD SVLAALSALP DSCDAWALVH DAARPCLTHG 

       130        140        150        160        170        180 
DIDALLASRL TYPQGAILAM PVRDTMKRAA TDGSIETTVC REALWHALTP QLFPAERLKQ 

       190        200        210        220        230 
HLEQALAAGV SITDEASAME WAGVYPGLVS GRADNIKVTH PDDLQLAGLF LQAQQQHT

« Hide

References

[1]"Complete sequence of Shewanella amazonensis SB2B."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000507 Genomic DNA. Translation: ABL99245.1.
RefSeqYP_926915.1. NC_008700.1.

3D structure databases

ProteinModelPortalA1S4D9.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1S4D9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4603290.
GenomeReviewsGene locus Sama_1038 in contig CP000507_GR.
KEGGsaz:Sama_1038.
PATRIC23450687. VBISheAma74963_1072.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1211.
HOGENOMHBG672839.
OMAFPQGAIL.
PhylomeDBA1S4D9.
ProtClustDBPRK00155.

Family and domain databases

HAMAPMF_00108. IspD.
[Tree]
InterProIPR001228. ISPD_synthase.
[Graphical view]
KOK00991.
PfamPF01128. IspD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00453. IspD. 1 hit.
PROSITEPS01295. ISPD. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPD_SHEAM
AccessionPrimary (citable) accession number: A1S4D9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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