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A1S462 (IF2_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:Sama_0961
OrganismShewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]
Taxonomic identifier326297 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length882 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 882882Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000008327

Regions

Nucleotide binding391 – 3988GTP By similarity
Nucleotide binding437 – 4415GTP By similarity
Nucleotide binding491 – 4944GTP By similarity
Region385 – 533149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
A1S462 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: FBB2AA4E6C2155CF

FASTA88295,960
        10         20         30         40         50         60 
MTEITVEKLA TEVGKTVDRL IEQFAQAGIK KAKADTVSES EKQQLLDFLK KQHGADAQPT 

        70         80         90        100        110        120 
KMTLQRKTVS TLSVSSGGGQ SKDVKVEVRK KRTFVKRDGN EAALKAEEEA RAQAEAQAKA 

       130        140        150        160        170        180 
EAEAKAKAEA EAKAKADAEA KAKAKAEAEA KAKASASAAK EQPKPVESEE AKAEAARLKS 

       190        200        210        220        230        240 
QQEEAAKSKA AQEEAAAKEK ARLLAEENAA RWAEEERRRI EAERYGDHHV TTSKVARAAE 

       250        260        270        280        290        300 
DSADLDDEKR GRRNRNKTQT KSKRGGKDAR EGREKHMKYK STPESMAHGF NKPVAAVTRD 

       310        320        330        340        350        360 
VRIGETVTVA ELAQKMAVKA TEIIKAMMKM GSMVTINQVL DQETAQLVAE EMGHKVVLLR 

       370        380        390        400        410        420 
ENELEHQVLA DRDDEGTTKL EPRAPVVTIM GHVDHGKTSL LDYIRRTKVA AGEAGGITQH 

       430        440        450        460        470        480 
IGAYHVETDN GMITFLDTPG HAAFTAMRAR GAKATDIVIL VVAADDGVMP QTIEAIQHAK 

       490        500        510        520        530        540 
AGNVPLIVAV NKMDKPEADI DRVKNELSQH GVMSEDWGGE NMFCYVSAKT GQGVDELLEA 

       550        560        570        580        590        600 
ILLQAEVLEL KAVRDGMAAG VVIESQLDKG RGPVATVLVQ EGTLRQGDIV LCGLEYGKIR 

       610        620        630        640        650        660 
AMKDENGRPI MEAGPSIPVE ILGLSGVPSA GDEATVVRDE RKAREVALYR QGKFRDVKLA 

       670        680        690        700        710        720 
RQQKSKLENM FANMTEGEVQ ELNIVLKADV QGSLEAITDS LRKLSTDEVK VNIIASGVGA 

       730        740        750        760        770        780 
LTETDATLAA ASNAIMVGFN VRADAQARKT IESEAVDLRY YSVIYDLIDE VKSAMSGMLS 

       790        800        810        820        830        840 
PEFKQQIIGL AEVRDVFKSP KLGAIAGCMV IEGIVKRSAP IRVLRENVVI YEGELESLRR 

       850        860        870        880 
FKDDVNEVRN GMECGIGVKN YNDVRVGDQI EVFETIEVAR TL 

« Hide

References

[1]"Complete sequence of Shewanella amazonensis SB2B."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000507 Genomic DNA. Translation: ABL99168.1.
RefSeqYP_926838.1. NC_008700.1.

3D structure databases

ProteinModelPortalA1S462.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326297.Sama_0961.

Proteomic databases

PRIDEA1S462.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL99168; ABL99168; Sama_0961.
GeneID4603213.
KEGGsaz:Sama_0961.
PATRIC23450535. VBISheAma74963_0996.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMANFECGLS.
OrthoDBEOG67HJSV.

Enzyme and pathway databases

BioCycSAMA326297:GH0T-996-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_SHEAM
AccessionPrimary (citable) accession number: A1S462
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries