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A1S425

- SYI_SHEAM

UniProt

A1S425 - SYI_SHEAM

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSAMA326297:GH0T-955-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Sama_0924
OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Taxonomic identifieri326297 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000009175: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000022115Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi326297.Sama_0924.

Structurei

3D structure databases

ProteinModelPortaliA1S425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1S425-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKSTLNL PETEFPMRGN LANREPAMLE RWNKDKLYQQ IRDSRIGRKP
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIELKVEQKV GKPGQKISAA EFREECRKYA AAQVDGQRED FIRLGVLGDW
160 170 180 190 200
HKPYLTMDFA TEANIVRSLA KVISNGHLQK GVKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYEDKTSPAI DVGFNVVDKP ALLAKFGVAQ YNHDIAMVIW TTTPWTLPAN
260 270 280 290 300
RALAIAADLE YVLVSFTKED VTRAIVVADV LHEDCVKRFG AESFEVLGRI
310 320 330 340 350
KGSELELTRF AHPFLDFDVP VILGDHVTTD AGTGVVHTAP GHGQDDFVVG
360 370 380 390 400
QKYGLEVANP VGDNGVYKAD TPFFAGQHVF KANDNVVALL KEKGALLNHV
410 420 430 440 450
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN QGLRSTALSE IQNTQWIPDW
460 470 480 490 500
GQSRIETMVA NRPDWCISRQ RTWGVPITLF VNKENEELHP NSVSLMERVA
510 520 530 540 550
NRIEQHGIQA WWDLDAAELL GDEAEQYRKV TDTLDVWYDS GSTFETVVAA
560 570 580 590 600
RPEFQGHGVD LYLEGSDQHR GWFMSSLMLS TAMHAKAPYK QVLTHGFTVD
610 620 630 640 650
GKGRKMSKSI GNVIAPQEVT NKLGADILRL WVAATDYSGE MSVSDEILNR
660 670 680 690 700
AADSYRRIRN TGRFLLANLN GFEPEADMVA VADMVALDRW MVRRAAKVQS
710 720 730 740 750
EIIAAYEQYN FHLVTHKLMQ FCSVELGSFY LDIIKDRQYT AKRESHARRS
760 770 780 790 800
CQSALFHIAE AMVRWIAPVL SFTADEIWQL LPGKREAYVF TQEWYEGLES
810 820 830 840 850
ITLESDLADS HWELLLSVRN EVNKELEQAR RDKVLGGSLE ATVTLYADTE
860 870 880 890 900
LAAKVAVLGD ELRFVLLTSD AKVLPIDAAP ESAVATELAG LKVLVAKTEA
910 920 930 940
AKCERCWHHR EDVGSVEAHP SLCGRCVTNI EGEGEARAFA
Length:940
Mass (Da):105,272
Last modified:February 6, 2007 - v1
Checksum:iD7D35AE30A62C327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000507 Genomic DNA. Translation: ABL99131.1.
RefSeqiWP_011759040.1. NC_008700.1.
YP_926801.1. NC_008700.1.

Genome annotation databases

EnsemblBacteriaiABL99131; ABL99131; Sama_0924.
GeneIDi4603176.
KEGGisaz:Sama_0924.
PATRICi23450455. VBISheAma74963_0960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000507 Genomic DNA. Translation: ABL99131.1 .
RefSeqi WP_011759040.1. NC_008700.1.
YP_926801.1. NC_008700.1.

3D structure databases

ProteinModelPortali A1S425.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326297.Sama_0924.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL99131 ; ABL99131 ; Sama_0924 .
GeneIDi 4603176.
KEGGi saz:Sama_0924.
PATRICi 23450455. VBISheAma74963_0960.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SAMA326297:GH0T-955-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1098 / SB2B.

Entry informationi

Entry nameiSYI_SHEAM
AccessioniPrimary (citable) accession number: A1S425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3