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A1S425

- SYI_SHEAM

UniProt

A1S425 - SYI_SHEAM

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Sama_0924
Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSAMA326297:GH0T-955-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Sama_0924
OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Taxonomic identifieri326297 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000009175: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligaseUniRule annotationPRO_1000022115Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi326297.Sama_0924.

Structurei

3D structure databases

ProteinModelPortaliA1S425.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1S425-1 [UniParc]FASTAAdd to Basket

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MSDYKSTLNL PETEFPMRGN LANREPAMLE RWNKDKLYQQ IRDSRIGRKP    50
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKTLAGFDAP YVPGWDCHGL 100
PIELKVEQKV GKPGQKISAA EFREECRKYA AAQVDGQRED FIRLGVLGDW 150
HKPYLTMDFA TEANIVRSLA KVISNGHLQK GVKPVHWCTD CGSALAEAEV 200
EYEDKTSPAI DVGFNVVDKP ALLAKFGVAQ YNHDIAMVIW TTTPWTLPAN 250
RALAIAADLE YVLVSFTKED VTRAIVVADV LHEDCVKRFG AESFEVLGRI 300
KGSELELTRF AHPFLDFDVP VILGDHVTTD AGTGVVHTAP GHGQDDFVVG 350
QKYGLEVANP VGDNGVYKAD TPFFAGQHVF KANDNVVALL KEKGALLNHV 400
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN QGLRSTALSE IQNTQWIPDW 450
GQSRIETMVA NRPDWCISRQ RTWGVPITLF VNKENEELHP NSVSLMERVA 500
NRIEQHGIQA WWDLDAAELL GDEAEQYRKV TDTLDVWYDS GSTFETVVAA 550
RPEFQGHGVD LYLEGSDQHR GWFMSSLMLS TAMHAKAPYK QVLTHGFTVD 600
GKGRKMSKSI GNVIAPQEVT NKLGADILRL WVAATDYSGE MSVSDEILNR 650
AADSYRRIRN TGRFLLANLN GFEPEADMVA VADMVALDRW MVRRAAKVQS 700
EIIAAYEQYN FHLVTHKLMQ FCSVELGSFY LDIIKDRQYT AKRESHARRS 750
CQSALFHIAE AMVRWIAPVL SFTADEIWQL LPGKREAYVF TQEWYEGLES 800
ITLESDLADS HWELLLSVRN EVNKELEQAR RDKVLGGSLE ATVTLYADTE 850
LAAKVAVLGD ELRFVLLTSD AKVLPIDAAP ESAVATELAG LKVLVAKTEA 900
AKCERCWHHR EDVGSVEAHP SLCGRCVTNI EGEGEARAFA 940
Length:940
Mass (Da):105,272
Last modified:February 6, 2007 - v1
Checksum:iD7D35AE30A62C327
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000507 Genomic DNA. Translation: ABL99131.1.
RefSeqiWP_011759040.1. NC_008700.1.
YP_926801.1. NC_008700.1.

Genome annotation databases

EnsemblBacteriaiABL99131; ABL99131; Sama_0924.
GeneIDi4603176.
KEGGisaz:Sama_0924.
PATRICi23450455. VBISheAma74963_0960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000507 Genomic DNA. Translation: ABL99131.1 .
RefSeqi WP_011759040.1. NC_008700.1.
YP_926801.1. NC_008700.1.

3D structure databases

ProteinModelPortali A1S425.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326297.Sama_0924.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL99131 ; ABL99131 ; Sama_0924 .
GeneIDi 4603176.
KEGGi saz:Sama_0924.
PATRICi 23450455. VBISheAma74963_0960.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SAMA326297:GH0T-955-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1098 / SB2B.

Entry informationi

Entry nameiSYI_SHEAM
AccessioniPrimary (citable) accession number: A1S425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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