Reviewed, UniProtKB/Swiss-Prot A1S3J7 (KATG2_SHEAM)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	Sama_0745

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 738	712	Catalase-peroxidase 2 HAMAP MF_01961		PRO_5000205037
Sites
	Active site	105	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	101	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	104 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-104) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1S3J7-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 16A56208A6DDEC69	FASTA	738	80,792
	10 20 30 40 50 60 MKRTLPTLSA LALSMSLALA AGQTQAAETT TNSYWWPEQL DLSPLRQHGS ESNPYGADYD 70 80 90 100 110 120 YAKAFGSLDL VAVKADIKTV LTTSQDWWPA DYGHYGPFFI RMAWHSAGVY RIFDGRGGAS 130 140 150 160 170 180 GGQQRFEPLN SWPDNVNLDK ARRLLWPIKQ KYGSKISWAD LMVLAGNVSL ESMGFKTFGF 190 200 210 220 230 240 AGGRTDDWEA ERVDWGHEKK WLDNKRHNEK GELAKPLGAT QMGLIYVNPE GPNGVPDPLA 250 260 270 280 290 300 AARDIRETFG RMAMNDEETV ALIAGGHTFG KAHGAHDPAK CVGADPAAAG VEAQGLGWKN 310 320 330 340 350 360 KCGKGHSEDT VTSGLEGAWS VSPTQWTMQY LDNLYGFEWV QSKSPAGHIQ WIPANGAGDN 370 380 390 400 410 420 LVPDAHVAGK RHAPIMFTTD IALKEDPSYR EITSRFRKDP KAFELAFAKA WFKLTHRDMG 430 440 450 460 470 480 PKTRYLGSDV PAEALIWQDP IPALDHAVID TNDIAALKSA ILASGIAVPE LVRTAWASAA 490 500 510 520 530 540 SFRGTDMRGG ANGARLRLEP QRSWEVNNPE ELAKVLPKLE KIQKDFNKSL KGGKKVSLAD 550 560 570 580 590 600 VIVLAGGTAI EQAARSAGYS VTVPFTPGRM DATTAQTDVS SFAVLEPKAD AFRNFFSEES 610 620 630 640 650 660 YLPPAEALVE KATQLTLSVP EMTALVGGLR VLDANSNKAK HGVFTDKPGN LSNDFFVNLL 670 680 690 700 710 720 DMSTRWEKSP REAGLYQGFD RQSGKLRWTA TPVDLIFGSH AELRAVAEVY GANDGKDRFV 730 EDFIAAWTKV MNLDRFDI

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000507 Genomic DNA. Translation: ABL98953.1.
RefSeq	YP_926623.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1S3J7.
Genome annotation databases
GeneID	4602998.
GenomeReviews	Gene locus Sama_0745 in contig CP000507_GR.
KEGG	saz:Sama_0745.
NMPDR	fig|326297.7.peg.697.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GTDMRGG.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_SHEAM

Accession

Primary (citable) accession number: A1S3J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)