A1S2E6 (GLPK_SHEAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Sama_0341

Organism

Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) [Complete proteome] [HAMAP]

Taxonomic identifier

326297 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Glycerol kinase HAMAP MF_00186		PRO_1000020777
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1S2E6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C0BDF3BA2CF26D74	FASTA	495	53,754
	10 20 30 40 50 60 MDKQYVVALD QGTTSSRAIV FDRDANLVAV SQREFTQIYP KGGWVEHDPM EIWASQSSTL 70 80 90 100 110 120 IEVLARAGIH SDEVAAIGIT NQRETTIIWD KLTGKPVCNA IVWQCRRSAA ICEALKAQGL 130 140 150 160 170 180 EPLFRDKTGL LLDPYFSGTK IKWILDNVPG VRERADAGEL LFGTVDTWLV WKLTEGKVHV 190 200 210 220 230 240 TDPTNASRTL MYNIHSGEWD SELLKALDIP ASLLPEVKSS QAVYGTTRIA GEGTEIPVAG 250 260 270 280 290 300 MAGDQQAALF GQLCVEPGMA KNTYGTGCFL LMNTGKQAVK SEHGLLTTIA VGDDGGISYA 310 320 330 340 350 360 LEGAVFMGGA TIQWLRDELG LIRDASDTEY FASKVDDTNG VYLVPAFVGL GAPYWDPDAR 370 380 390 400 410 420 GTLVGLTRGA NRNHIIRAAL ESIAYQSRDL LDAMSKDSGV ALKQLKVDGG AVANDFLMQF 430 440 450 460 470 480 QADISGVEVL RPAQTETTAM GAAFLGGLAV GFWSNVDEIR HKSGIDRRFV PLMDDKERAT 490 LYLGWQDAVR RSLSS

References

[1]

"Complete sequence of Shewanella amazonensis SB2B." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1098 / SB2B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000507 Genomic DNA. Translation: ABL98552.1.
RefSeq	YP_926222.1. NC_008700.1.
3D structure databases
ProteinModelPortal	A1S2E6.
SMR	A1S2E6. Positions 3-494.
ModBase	Search...
Protein-protein interaction databases
STRING	A1S2E6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4602597.
GenomeReviews	Gene locus Sama_0341 in contig CP000507_GR.
KEGG	saz:Sama_0341.
NMPDR	fig|326297.7.peg.324.
PATRIC	23449217. VBISheAma74963_0351.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	ADTIGMP.
PhylomeDB	A1S2E6.
ProtClustDB	CLSK907562.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_SHEAM

Accession

Primary (citable) accession number: A1S2E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program