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A1S1I8

- FADB_SHEAM

UniProt

A1S1I8 - FADB_SHEAM

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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei118 – 1181Important for catalytic activityUniRule annotation
Sitei138 – 1381Important for catalytic activityUniRule annotation
Binding sitei295 – 2951SubstrateUniRule annotation
Binding sitei323 – 3231NAD; via amide nitrogenUniRule annotation
Binding sitei342 – 3421NADUniRule annotation
Binding sitei406 – 4061NADUniRule annotation
Active sitei449 – 4491For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation
Binding sitei452 – 4521NADUniRule annotation
Binding sitei499 – 4991SubstrateUniRule annotation
Binding sitei659 – 6591SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi399 – 4013NADUniRule annotation
Nucleotide bindingi426 – 4283NADUniRule annotation

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. coenzyme binding Source: InterPro
  4. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB-HAMAP
  5. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSAMA326297:GH0T-32-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:Sama_0032
OrganismiShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
Taxonomic identifieri326297 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000009175: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. fatty acid beta-oxidation multienzyme complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Fatty acid oxidation complex subunit alphaPRO_1000069572Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi326297.Sama_0032.

Structurei

3D structure databases

ProteinModelPortaliA1S1I8.
SMRiA1S1I8. Positions 1-713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 188188Enoyl-CoA hydratase/isomeraseUniRule annotationAdd
BLAST
Regioni310 – 7164073-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261344.
KOiK01825.
OMAiNPIVVND.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01621. FadB.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1S1I8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIYQSPTIQV ELTADKIARL CFNAPGSVNK FDRETLASLN AALDVLKDSD
60 70 80 90 100
AKAAVLTSGK DTFIVGADIT EFLALFAEED AKLMEWIAQA NVVFNKLEDL
110 120 130 140 150
PFPTVSAIKG FALGGGCEAI LATDFRVADT SAKIGLPETK LGLIPGFGGT
160 170 180 190 200
VRLPRLIGAD NALEWITTGK DQRPEDALKV GAIDAVVAPE NLEAAAIQML
210 220 230 240 250
NDALAGKLDW QARRARKQAP LTLPKLEAMM SFTTAKGMVY AVAGKHYPAP
260 270 280 290 300
MAAVSVVEQA AGMSRAEALV VEHNAFIKLA KTDVATALIG IFLNDQLVKG
310 320 330 340 350
KAKKASKLAK DIKHAAVLGA GIMGGGIAYQ SASKGTPIVM KDINQAALDL
360 370 380 390 400
GVNEAAKLLS AQVARGRSTP DKMAKVLNNI TPALDYAPLK DVNVVVEAVV
410 420 430 440 450
ENPKVKAMVL ADVENVVADD AIIASNTSTI SIDLLAKSLK NPARFCGMHF
460 470 480 490 500
FNPVHKMPLV EVIRGKDTSE ETVASVVAYA SKMGKTPIVV NDCPGFFVNR
510 520 530 540 550
VLFPYFAGFN GLLADGGDFA AIDKVMEKQF GWPMGPAYLL DVVGLDTGHH
560 570 580 590 600
AQAVMADGFP DRMGKSDKDA IDVMYEAGRL GQKNGKGFYQ YSIDKRGKPK
610 620 630 640 650
KDVDPASYTM LAEAFGAQKA FEADEIIART MIPMIIETVR CLEEGIVASP
660 670 680 690 700
AEADMGLVYG LGFPPFRGGV FRYLDTMGVA NFVALADKYA HLGGLYQVTD
710
AMRELASNNG SYYPKA
Length:716
Mass (Da):76,369
Last modified:February 6, 2007 - v1
Checksum:i93251CB6B0C3D409
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000507 Genomic DNA. Translation: ABL98244.1.
RefSeqiYP_925914.1. NC_008700.1.

Genome annotation databases

EnsemblBacteriaiABL98244; ABL98244; Sama_0032.
GeneIDi4602289.
KEGGisaz:Sama_0032.
PATRICi23448535. VBISheAma74963_0031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000507 Genomic DNA. Translation: ABL98244.1 .
RefSeqi YP_925914.1. NC_008700.1.

3D structure databases

ProteinModelPortali A1S1I8.
SMRi A1S1I8. Positions 1-713.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326297.Sama_0032.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL98244 ; ABL98244 ; Sama_0032 .
GeneIDi 4602289.
KEGGi saz:Sama_0032.
PATRICi 23448535. VBISheAma74963_0031.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261344.
KOi K01825.
OMAi NPIVVND.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci SAMA326297:GH0T-32-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01621. FadB.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02437. FadB. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1098 / SB2B.

Entry informationi

Entry nameiFADB_SHEAM
AccessioniPrimary (citable) accession number: A1S1I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: October 1, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3