A1S061 (A1S061_THEPD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase 2 HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE2 HAMAP MF_00193
Ordered Locus Names:	Tpen_1444

Organism

Thermofilum pendens (strain Hrk 5)

Taxonomic identifier

368408 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermofilaceae › Thermofilum

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. RuleBase RU003812 HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL ABL78841.1
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	37 – 44	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	39	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A1S061 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C169AA1F7F0E27BE	FASTA	279	31,556
	10 20 30 40 50 60 MRYVLPPINA ERVAEYIARR LRWYVREYAG KRAGVVGVSG GVDSAVVAFL TARALGPENT 70 80 90 100 110 120 YCYVLPSFAT PKEDVEDALR VIEALGLPDG NWEVISVDPI LKSFEEVLGE MDRVARGNVM 130 140 150 160 170 180 ARIRMIILHE KAYAHNALVI GTGDKSELLL GYFTKYGDGG VDVLPIGGLY KTHVRQLARY 190 200 210 220 230 240 LGVPERIVEK PSSPRLWPGQ TAEGELGAPY ELVDSVLYLR FEKWLPEERI AEELGVEVGV 250 260 270 VKRILEMVKR SQHKRMMPEV FHVGQRDLGS DWRYPRQWF

References

[1]

"Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction." Anderson I., Rodriguez J., Susanti D., Porat I., Reich C., Ulrich L.E., Elkins J.G., Mavromatis K., Lykidis A., Kim E., Thompson L.S., Nolan M., Land M., Copeland A., Lapidus A., Lucas S., Detter C., Zhulin I.B. , Olsen G.J., Whitman W., Mukhopadhyay B., Bristow J., Kyrpides N. J. Bacteriol. 190:2957-2965(2008) [PubMed: 18263724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hrk 5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000505 Genomic DNA. Translation: ABL78841.1.
RefSeq	YP_920844.1. NC_008698.1.
3D structure databases
ProteinModelPortal	A1S061.
ModBase	Search...
Protein-protein interaction databases
STRING	A1S061.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4601153.
GenomeReviews	Gene locus Tpen_1444 in contig CP000505_GR.
KEGG	tpe:Tpen_1444.
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	VNIGPAV.
PhylomeDB	A1S061.
ProtClustDB	CLSK781705.
Enzyme and pathway databases
BioCyc	TPEN368408:TPEN_1444-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1S061_THEPD

Accession

Primary (citable) accession number: A1S061

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)