Reviewed, UniProtKB/Swiss-Prot A1RYE4 (GYAR_THEPD)

Names and origin

Protein names

Recommended name:
    Glyoxylate reductase
    EC=1.1.1.26

Gene names

Name:	gyaR
Ordered Locus Names:	Tpen_0823

Organism

Thermofilum pendens (strain Hrk 5) [Complete proteome] [HAMAP]

Taxonomic identifier

368408 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermofilaceae › Thermofilum

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Glycolate + NAD+ = glyoxylate + NADH. HAMAP MF_00776
Subunit structure	Homodimer By similarity. HAMAP MF_00776
Subcellular location	Cytoplasm By similarity HAMAP MF_00776.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Glyoxylate reductase HAMAP MF_00776		PRO_0000348416
Regions
	Nucleotide binding	157 – 160	4	NADP By similarity
	Nucleotide binding	239 – 241	3	NADP By similarity
	Nucleotide binding	289 – 291	3	NADP By similarity
Sites
	Active site	241	1	By similarity
	Active site	270	1	By similarity
	Active site	289	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1RYE4-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 83C1C68D2F9A7E5C	FASTA	339	37,993
	10 20 30 40 50 60 MARPKVYVTR IIPEPGLSML KECCDVVVHE SKDWPPSREE LLRNIRDKDA LLCLLTDKID 70 80 90 100 110 120 AEVMDAAPNL KVISTYSVGF DHIDIPEATK RGIYVTHTPG VLTDAVAEFT VGLILAVTRR 130 140 150 160 170 180 IVEADKIIRT GQWDKPWNPY FLTGPELKGK TIGLVGLGRI GVATAKRLSS FDVKILYYDI 190 200 210 220 230 240 ERRWDVETVI PNMEFTDLDT LLEKSDIVSI HVPLTKETYH LINEERLRKM KKTAYLINTA 250 260 270 280 290 300 RGPVVDTEAL VKALKEGWIA GAALDVFEQE PLPPNHPLTK FDNVVLAPHI ASATIEARQR 310 320 330 MAELAARNLI AVLKGEMPPA LVNKEVLKVR PLEKVKMIP

References

[1]

"Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction." Anderson I., Rodriguez J., Susanti D., Porat I., Reich C., Ulrich L.E., Elkins J.G., Mavromatis K., Lykidis A., Kim E., Thompson L.S., Nolan M., Land M., Copeland A., Lapidus A., Lucas S., Detter C., Zhulin I.B. , Olsen G.J., Whitman W., Mukhopadhyay B., Bristow J., Kyrpides N. J. Bacteriol. 190:2957-2965(2008) [PubMed: 18263724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000505 Genomic DNA. Translation: ABL78224.1.
RefSeq	YP_920227.1.
3D structure databases
SMR	A1RYE4. Positions 3-331.
ModBase	Search...
Protein-protein interaction databases
STRING	A1RYE4.
Genome annotation databases
GeneID	4601826.
GenomeReviews	Gene locus Tpen_0823 in contig CP000505_GR.
KEGG	tpe:Tpen_0823.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04496.
HOGENOM	HBG731446.
OMA	TEPRSAR.
PhylomeDB	A1RYE4.
Family and domain databases
HAMAP	MF_00776. GyaR. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYAR_THEPD

Accession

Primary (citable) accession number: A1RYE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	January 19, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)