A1RYD8 (SYP_THEPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Tpen_0817

Organism

Thermofilum pendens (strain Hrk 5)

Taxonomic identifier

368408 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermofilaceae › Thermofilum

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Proline--tRNA ligase HAMAP MF_01571		PRO_0000288424

Sequences

	Sequence		Length	Mass (Da)
	A1RYD8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: DE03235CC18F4C5E	FASTA	482	56,335
	10 20 30 40 50 60 MSEQGITVSK SEDFSEWYSQ VLSKAGLVDL RYNVQGFVVH KPWLMRIIKA IYRFFEEELE 70 80 90 100 110 120 KTGHEPVLFP LVIPEENFEK EKEHVEGFKP EVFWVTQAGD EKLERRLALR PTSETAFYYM 130 140 150 160 170 180 YSYWIQSWRD LPLKLYQSVS VYRNEKNTRP LIRGREFLWI EAHDAFATHE EALNQIREDM 190 200 210 220 230 240 ENSRKVIWEK LGIPFLFLRR PPWDKFSGAE DTYAADTIMP DGRVLQISST HDLGQRFAKA 250 260 270 280 290 300 FNVTFLDKDG KRKYVWQTCY GPGIWRITAA LIAIHGDDKG LVLPMNVAPI QVVIVPIYYK 310 320 330 340 350 360 ESDKERVLEK CRKLEAMIRE AGYRVYLDAR EEYTPGWKFN DWELKGVPVR LEVGVREVET 370 380 390 400 410 420 GTVTVFRRDL RVKEKVADSE LISHIRKLEN DILEELKRRA KEFFESRIVT ATRREEVEEA 430 440 450 460 470 480 LRSGKMVKMP FCGREECADD LKEATDGGKV RGTEIDFKEG DYGRCAWCGA PARLIVYVAK SY

References

[1]

"Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction." Anderson I., Rodriguez J., Susanti D., Porat I., Reich C., Ulrich L.E., Elkins J.G., Mavromatis K., Lykidis A., Kim E., Thompson L.S., Nolan M., Land M., Copeland A., Lapidus A., Lucas S., Detter C., Zhulin I.B. , Olsen G.J., Whitman W., Mukhopadhyay B., Bristow J., Kyrpides N. J. Bacteriol. 190:2957-2965(2008) [PubMed: 18263724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hrk 5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000505 Genomic DNA. Translation: ABL78218.1.
RefSeq	YP_920221.1. NC_008698.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1RYD8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4601988.
GenomeReviews	Gene locus Tpen_0817 in contig CP000505_GR.
KEGG	tpe:Tpen_0817.
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	A1RYD8.
Enzyme and pathway databases
BioCyc	TPEN368408:TPEN_0817-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_THEPD

Accession

Primary (citable) accession number: A1RYD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program