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Protein

DNA ligase 2

Gene

lig2

Organism
Thermofilum pendens (strain Hrk 5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei263 – 2631ATPUniRule annotation
Active sitei265 – 2651N6-AMP-lysine intermediateUniRule annotation
Binding sitei270 – 2701ATPUniRule annotation
Binding sitei285 – 2851ATPUniRule annotation
Binding sitei314 – 3141ATPUniRule annotation
Binding sitei354 – 3541ATPUniRule annotation
Binding sitei432 – 4321ATPUniRule annotation
Binding sitei438 – 4381ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTPEN368408:GHSG-797-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 2UniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 2UniRule annotation
Gene namesi
Name:lig2UniRule annotation
Ordered Locus Names:Tpen_0750
OrganismiThermofilum pendens (strain Hrk 5)
Taxonomic identifieri368408 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermofilaceaeThermofilum
ProteomesiUP000000641 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601DNA ligase 2PRO_0000365266Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi368408.Tpen_0750.

Structurei

3D structure databases

ProteinModelPortaliA1RY72.
SMRiA1RY72. Positions 7-595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiLACYDEQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1RY72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAAGDLPYS VLADFYERIE STSSRLAMTD YLVALFKKTP PEVIDKVVYL
60 70 80 90 100
TQGQLRPDYE GVELGVAEKL ALRALAKASG RHLKDVEDLY KKTGDIGAVA
110 120 130 140 150
EKLLTAPGGG LLEFFGTPVQ KKELTVSQVY SALMKIAQAS GEGAQETKVN
160 170 180 190 200
TLVALLQDAK PKEARYILRT VLGRLRLGIA DMTILDALAA AFAGSKAARD
210 220 230 240 250
VIERAYTKHP DLGYIAKLLA TKGLDAVASL KIEVGIPVLP MLAERLSDPA
260 270 280 290 300
EILEKLGGKC LAEYKYDGER VQAHKSGDKV LLFSRRLENI THHYPDVVEY
310 320 330 340 350
VKRLKVREAI VEGEIVAYNP DTGEMLPFQE LMHRRRKYDV EKAMKEYPVR
360 370 380 390 400
VYLFDVIYMD GEELIEKPLD QRRLILEKIV PEGDEDILLS TAKVVGDAKD
410 420 430 440 450
LLHFFEQAIS EGCEGVMCKS IGPGSIYQMG ARGWLWIKFK RDYRMEMTDT
460 470 480 490 500
VDLVVVGGFH GRGKRAGTYG ALLMAAYDPE TDTFKTVCKV GTGFTDEDLA
510 520 530 540 550
KLPELLDPYK IPHRHPRVFS KIEADVWFVP AVVLEIIGAE ITLSPLHTCA
560 570 580 590 600
LNKLEEGAGL AIRFPRFTGR YRFDKKPEQA TTESELIEMY KSQKKTALQQ

S
Length:601
Mass (Da):66,943
Last modified:February 5, 2007 - v1
Checksum:i8AA565878E7AF78B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000505 Genomic DNA. Translation: ABL78152.1.
RefSeqiYP_920155.1. NC_008698.1.

Genome annotation databases

EnsemblBacteriaiABL78152; ABL78152; Tpen_0750.
GeneIDi4600401.
KEGGitpe:Tpen_0750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000505 Genomic DNA. Translation: ABL78152.1.
RefSeqiYP_920155.1. NC_008698.1.

3D structure databases

ProteinModelPortaliA1RY72.
SMRiA1RY72. Positions 7-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi368408.Tpen_0750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL78152; ABL78152; Tpen_0750.
GeneIDi4600401.
KEGGitpe:Tpen_0750.

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiLACYDEQ.

Enzyme and pathway databases

BioCyciTPEN368408:GHSG-797-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hrk 5.

Entry informationi

Entry nameiDNLI2_THEPD
AccessioniPrimary (citable) accession number: A1RY72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2009
Last sequence update: February 5, 2007
Last modified: February 3, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.