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A1RY32 (TFE_THEPD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Transcription factor E

Short name=TFE
Alternative name(s):
TFIIE subunit alpha homolog
Transcription initiation factor TFIIE
Gene names
Name:tfe
Ordered Locus Names:Tpen_0710
OrganismThermofilum pendens (strain Hrk 5) [Reference proteome] [HAMAP]
Taxonomic identifier368408 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermofilaceaeThermofilum

Protein attributes

Sequence length178 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes By similarity. HAMAP-Rule MF_01909

Subunit structure

Monomer By similarity. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes By similarity. HAMAP-Rule MF_01909

Domain

The winged helix domain is involved in binding to DNA in the preinitiation complex By similarity. HAMAP-Rule MF_01909

Sequence similarities

Belongs to the TFE family.

Contains 1 HTH TFE/IIEalpha-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 178178Transcription factor E HAMAP-Rule MF_01909
PRO_0000326625

Regions

Domain3 – 8684HTH TFE/IIEalpha-type

Sequences

Sequence LengthMass (Da)Tools
A1RY32 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 3515BAD4F3F757D8

FASTA17820,575
        10         20         30         40         50         60 
MNAHEALAEI AGKMFGDNAK KVMMVLASNV EVSDEDLAKD LKMDPPELRK LLNDLFEARL 

        70         80         90        100        110        120 
VKYRRARDEN IGWYKYYWRI TDEPIQQILS DRKRLTLSIL EKVLSLEENS ETYVCPKCGT 

       130        140        150        160        170 
RYTVDEAENN NYTCEVCGEV LEPFDNTHRV EKLKRAIELL GNWDPSPKQQ ASRAIPHA 

« Hide

References

[1]"Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction."
Anderson I., Rodriguez J., Susanti D., Porat I., Reich C., Ulrich L.E., Elkins J.G., Mavromatis K., Lykidis A., Kim E., Thompson L.S., Nolan M., Land M., Copeland A., Lapidus A., Lucas S., Detter C., Zhulin I.B. expand/collapse author list , Olsen G.J., Whitman W., Mukhopadhyay B., Bristow J., Kyrpides N.
J. Bacteriol. 190:2957-2965(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hrk 5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000505 Genomic DNA. Translation: ABL78112.1.
RefSeqYP_920115.1. NC_008698.1.

3D structure databases

ProteinModelPortalA1RY32.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING368408.Tpen_0710.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL78112; ABL78112; Tpen_0710.
GeneID4601591.
KEGGtpe:Tpen_0710.

Phylogenomic databases

eggNOGCOG1675.
KOK03136.
OMADSGWLTY.

Enzyme and pathway databases

BioCycTPEN368408:GHSG-756-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
HAMAPMF_01909. TFE_arch.
InterProIPR016481. TF_E_archaea.
IPR017919. TFIIE/TFIIEa_HTH.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamPF08271. TF_Zn_Ribbon. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
PROSITEPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTFE_THEPD
AccessionPrimary (citable) accession number: A1RY32
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families