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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Thermofilum pendens (strain Hrk 5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathway:iL-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (mtnP)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121PhosphateUniRule annotation
Sitei167 – 1671Important for substrate specificityUniRule annotation
Binding sitei185 – 1851Substrate; via amide nitrogenUniRule annotation
Binding sitei186 – 1861PhosphateUniRule annotation
Sitei220 – 2201Important for substrate specificityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciTPEN368408:GHSG-657-MONOMER.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:Tpen_0618
OrganismiThermofilum pendens (strain Hrk 5)
Taxonomic identifieri368408 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermofilaceaeThermofilum
ProteomesiUP000000641 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262S-methyl-5'-thioadenosine phosphorylasePRO_0000415110Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi368408.Tpen_0618.

Structurei

3D structure databases

ProteinModelPortaliA1RXU2.
SMRiA1RXU2. Positions 2-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 552Phosphate bindingUniRule annotation
Regioni87 – 882Phosphate bindingUniRule annotation
Regioni209 – 2113Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiMTNHTEA.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1RXU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVKIGIIG GSGLYSPDFL TNPKEEKIYT PYGPPSSHVV IGEIAGRKVA
60 70 80 90 100
FIPRHGKRHE IPPHKVNYRA NIYALKELGV ERLISVSAVG SLREDYKPGD
110 120 130 140 150
FVCTDQFIDM TKGRVYTFYD GPVVAHVSMA DPFCPELREL CIRSARKLGI
160 170 180 190 200
TMHEKGTYIC IEGPRFSTRA ESRLWRQFGA DIIGMTLVPE VNLAREARMC
210 220 230 240 250
FLNIAMVTDY DVWAEKPVTA HEVARVMAEN TEKVKRLLAD LIPSIPEERK
260
CQCARALDEA LI
Length:262
Mass (Da):29,467
Last modified:February 6, 2007 - v1
Checksum:i0805BD30C4FB86E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000505 Genomic DNA. Translation: ABL78022.1.
RefSeqiWP_011752287.1. NC_008698.1.
YP_920025.1. NC_008698.1.

Genome annotation databases

EnsemblBacteriaiABL78022; ABL78022; Tpen_0618.
GeneIDi4601324.
KEGGitpe:Tpen_0618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000505 Genomic DNA. Translation: ABL78022.1.
RefSeqiWP_011752287.1. NC_008698.1.
YP_920025.1. NC_008698.1.

3D structure databases

ProteinModelPortaliA1RXU2.
SMRiA1RXU2. Positions 2-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi368408.Tpen_0618.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL78022; ABL78022; Tpen_0618.
GeneIDi4601324.
KEGGitpe:Tpen_0618.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiMTNHTEA.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.
BioCyciTPEN368408:GHSG-657-MONOMER.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hrk 5.

Entry informationi

Entry nameiMTAP_THEPD
AccessioniPrimary (citable) accession number: A1RXU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: February 6, 2007
Last modified: May 27, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.