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Protein

Flap endonuclease 1

Gene

fen

Organism
Thermofilum pendens (strain DSM 2475 / Hrk 5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).By similarity

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Magnesium 1UniRule annotation1
Metal bindingi82Magnesium 1UniRule annotation1
Metal bindingi154Magnesium 1UniRule annotation1
Metal bindingi156Magnesium 1UniRule annotation1
Metal bindingi175Magnesium 2UniRule annotation1
Metal bindingi177Magnesium 2UniRule annotation1
Metal bindingi238Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:Tpen_0302
OrganismiThermofilum pendens (strain DSM 2475 / Hrk 5)
Taxonomic identifieri368408 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermofilaceaeThermofilum
Proteomesi
  • UP000000641 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000613341 – 346Flap endonuclease 1Add BLAST346

Interactioni

Subunit structurei

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi368408.Tpen_0302.

Structurei

3D structure databases

ProteinModelPortaliA1RWY2.
SMRiA1RWY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 100N-domainAdd BLAST100
Regioni118 – 260I-domainAdd BLAST143
Regioni336 – 344Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
KOiK04799.
OMAiGSQDYDS.
OrthoDBiPOG093Z0642.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiView protein in InterPro
IPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiView protein in Pfam
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
PRINTSiPR00853. XPGRADSUPER.
SMARTiView protein in SMART
SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00841. XPG_1. 1 hit.

Sequencei

Sequence statusi: Complete.

A1RWY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVDIKELVE PVAKEVELGF FSKKVIAIDA YNSLYQFLAT IRQKDGTPLL
60 70 80 90 100
DAQGNVTSHL NGLFYRTINY IELGIKPVYV FDGRPPELKQ KELERRYQIK
110 120 130 140 150
VEAEKKYREA IERGDLEEAR IYAQQTSRLT AAMVHDAKLL LRYMGVPYVE
160 170 180 190 200
APSEGEAQAA YMVKKGDAWA SGSQDFDSLL FGSPRLVRNL AITGKRKLPR
210 220 230 240 250
KDVYVEVKPE IVELEELLRV HGITHQQLVV IGILVGTDYA PEGARGIGVK
260 270 280 290 300
KALKLVKELK DPEKIFRSVE WSSDVPPEKI LELFLHPEVT DSYELTWKEP
310 320 330 340
DKEKVIELLV ERHQFSMERV TNALDRLEKA VKTHFKQQSL ESWFGF
Length:346
Mass (Da):39,575
Last modified:February 6, 2007 - v1
Checksum:i00E7F92D87D7F88A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000505 Genomic DNA. Translation: ABL77712.1.
RefSeqiWP_011751977.1. NC_008698.1.

Genome annotation databases

EnsemblBacteriaiABL77712; ABL77712; Tpen_0302.
GeneIDi4601125.
KEGGitpe:Tpen_0302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000505 Genomic DNA. Translation: ABL77712.1.
RefSeqiWP_011751977.1. NC_008698.1.

3D structure databases

ProteinModelPortaliA1RWY2.
SMRiA1RWY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi368408.Tpen_0302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL77712; ABL77712; Tpen_0302.
GeneIDi4601125.
KEGGitpe:Tpen_0302.

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
KOiK04799.
OMAiGSQDYDS.
OrthoDBiPOG093Z0642.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiView protein in InterPro
IPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiView protein in Pfam
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
PRINTSiPR00853. XPGRADSUPER.
SMARTiView protein in SMART
SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00841. XPG_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFEN_THEPD
AccessioniPrimary (citable) accession number: A1RWY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: March 15, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.