Reviewed, UniProtKB/Swiss-Prot A1RWC8 (RIFK_THEPD)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Tpen_0098

Organism

Thermofilum pendens (strain Hrk 5) [Complete proteome] [HAMAP]

Taxonomic identifier

368408 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermofilaceae › Thermofilum

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Riboflavin kinase HAMAP MF_01285		PRO_0000322107
Regions
	Nucleotide binding	102 – 107	6	CDP By similarity
	Nucleotide binding	207 – 210	4	CDP By similarity
	Region	1 – 92	92	H-T-H motif-like HAMAP MF_01285
	Region	93 – 227	135	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	131	1	Magnesium By similarity
	Metal binding	133	1	Magnesium By similarity
	Binding site	194	1	FMN By similarity
	Binding site	202	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1RWC8-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3944F9974A920F75	FASTA	227	25,939
	10 20 30 40 50 60 MSKDYEVFPL LLEMAKRGCL VAPRRLSRIE ILKTLGLTPW RFKKLVEAAE EEGYIERRVH 70 80 90 100 110 120 GRMVFYVVTE RGRALLRRVY DDLKRTIDSS TFLTLRGYVV PGLGEGAIYM GIPRYVEAFK 130 140 150 160 170 180 EVLGYEPYPG TLNIKLVDED VYLRRALREK RVGFRIEGFR LDEGRESCGV TVYKAMIMAN 190 200 210 220 GVTVSGAALD IDKTKHGDEI LELIAPVRLR DELRLKDGDK VEVVIPV

References

[1]

"Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction." Anderson I., Rodriguez J., Susanti D., Porat I., Reich C., Ulrich L.E., Elkins J.G., Mavromatis K., Lykidis A., Kim E., Thompson L.S., Nolan M., Land M., Copeland A., Lapidus A., Lucas S., Detter C., Zhulin I.B. , Olsen G.J., Whitman W., Mukhopadhyay B., Bristow J., Kyrpides N. J. Bacteriol. 190:2957-2965(2008) [PubMed: 18263724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000505 Genomic DNA. Translation: ABL77508.1.
RefSeq	YP_919511.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1RWC8.
Genome annotation databases
GeneID	4602014.
GenomeReviews	Gene locus Tpen_0098 in contig CP000505_GR.
KEGG	tpe:Tpen_0098.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GAYYVRQ.
Family and domain databases
HAMAP	MF_01285. Fused. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_THEPD

Accession

Primary (citable) accession number: A1RWC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)