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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NAD; via amide nitrogenUniRule annotation1
Binding sitei60NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei124NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi154Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi169IronUniRule annotation1
Binding sitei219NAD; via amide nitrogenUniRule annotation1
Binding sitei229GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 53NADUniRule annotation2
Nucleotide bindingi152 – 154NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Pisl_1998
OrganismiPyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Taxonomic identifieri384616 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003222221 – 261Thiamine thiazole synthaseAdd BLAST261

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi384616.Pisl_1998.

Structurei

3D structure databases

ProteinModelPortaliA1RW13.
SMRiA1RW13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OrthoDBiPOG093Z09PU.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

A1RW13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKIARAII KHGLEDLYEY SDVDVAIVGA GPAGLTAARY LAERGHKVVV
60 70 80 90 100
YERRFSFGGG IGPGGNMIPK IVVQEEAVPV LRDFKIRYKP VGDGLYTVDP
110 120 130 140 150
AELIAKLAAG AIDAGAKIIL GVHVDDVIFR GDPPRVVGLL WVWTPIQMSG
160 170 180 190 200
SHVDPLYTQA KAVLDATGHD AEVISIAARK VPELGIEVRG EKSAWAEVSE
210 220 230 240 250
KLVVEHTGRV APGLYVAGMA VCTVHGLPRM GPIFGGMLLS GRRAAEIIHK
260
DLVEEVYAVR A
Length:261
Mass (Da):27,958
Last modified:February 26, 2008 - v2
Checksum:iBCB7BAE7E0284EEB
GO

Sequence cautioni

The sequence ABL89145 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL89145.1. Different initiation.
RefSeqiWP_053240513.1. NC_008701.1.

Genome annotation databases

EnsemblBacteriaiABL89145; ABL89145; Pisl_1998.
GeneIDi4617069.
KEGGipis:Pisl_1998.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL89145.1. Different initiation.
RefSeqiWP_053240513.1. NC_008701.1.

3D structure databases

ProteinModelPortaliA1RW13.
SMRiA1RW13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi384616.Pisl_1998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL89145; ABL89145; Pisl_1998.
GeneIDi4617069.
KEGGipis:Pisl_1998.

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OrthoDBiPOG093Z09PU.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_PYRIL
AccessioniPrimary (citable) accession number: A1RW13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: May 10, 2017
This is version 61 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.