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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84NucleophileUniRule annotation1
Binding sitei116L-glutamineUniRule annotation1
Active sitei184Charge relay systemUniRule annotation1
Active sitei186Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:Pisl_1336
OrganismiPyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Taxonomic identifieri384616 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002930231 – 202Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST202

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi384616.Pisl_1336.

Structurei

3D structure databases

ProteinModelPortaliA1RU67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 54L-glutamine bindingUniRule annotation3
Regioni143 – 144L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00034. Archaea.
COG0311. LUCA.
HOGENOMiHOG000039949.
KOiK08681.
OMAiVFIRAPI.
OrthoDBiPOG093Z0AY8.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
PANTHERiPTHR31559. PTHR31559. 1 hit.
PfamiView protein in Pfam
PF01174. SNO. 1 hit.
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiView protein in PROSITE
PS51130. PDXT_SNO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A1RU67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAGVLALQG DVEEHIQAFK KAAQELGISI EVIQVKKPQD LKEIATLAIP
60 70 80 90 100
GGESTTIGAL AKRTGLLDAL RDAIKEGLPT LGTCAGAIFL AKEVRDSVVG
110 120 130 140 150
ETKQPILGLM DIAVVRNAFG RQRESFEVDL QVGELGTVRA VFIRAPAFIK
160 170 180 190 200
AWGDARLAAP LKHPELGQIY AAAFQGHMVA TAFHPELSTT VVHKYMLNLA

KK
Length:202
Mass (Da):21,660
Last modified:February 6, 2007 - v1
Checksum:iB11F232C3393D0E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL88499.1.
RefSeqiWP_011763074.1. NC_008701.1.

Genome annotation databases

EnsemblBacteriaiABL88499; ABL88499; Pisl_1336.
GeneIDi4617484.
KEGGipis:Pisl_1336.

Similar proteinsi

Entry informationi

Entry nameiPDXT_PYRIL
AccessioniPrimary (citable) accession number: A1RU67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: February 6, 2007
Last modified: November 22, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families