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A1RTX9 (SYL_PYRIL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pisl_1246
OrganismPyrobaculum islandicum (strain DSM 4184 / JCM 9189) [Complete proteome] [HAMAP]
Taxonomic identifier384616 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length945 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 945945Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009405

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif638 – 6425"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1RTX9 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 66351DCA0CEB630D

FASTA945107,947
        10         20         30         40         50         60 
MSELSRFFIE IAERWQKRWK ESRVFEPEPT PGVAKYFITA AYPYPNGAVH IGHGRTYLIA 

        70         80         90        100        110        120 
DVLARFYRHM GRVVLYPMGF HYTGTPILTI AEVIAAGDKT VIEEYMEIYG VPEEEIKKMG 

       130        140        150        160        170        180 
DPLYLARYFH NRSKMAMERF GLSIDWTREF TTIDPEYQRF IQWQFEKLRK KGLIVRGRHP 

       190        200        210        220        230        240 
VGWCPRHSMP VGAHDTKDDK EPEIGQWTLI YFTDSEGLTF PAATLRPETV PGVTNLWINP 

       250        260        270        280        290        300 
DAEYVVAEFD GKRVVISRDA AYRLSFQAEV KILREARGRE FVGRSVQNPV TGEWVPIYEA 

       310        320        330        340        350        360 
WFVDPRVGTG VVMSVPAHAP YDYVALRDLG IEKLIPLIKV EGYGDYPAKE VVERMGIKSQ 

       370        380        390        400        410        420 
TDPALEEATK EVYSTEYTRG VMREDVTERI GIHLQEPARS MLRAVFKMYF AGRPVREARE 

       430        440        450        460        470        480 
FIAKWLVEAR LGGVMYDIMN KPVYCRCGTE IVVKVLEDQW FINYGDPKWK ETARKLVDEM 

       490        500        510        520        530        540 
VIIPGEAKAH FFATIDWLDK RACARTRGLG TPLPWSSGWI IESLSDSTIY MAFYTVIKKI 

       550        560        570        580        590        600 
RQFGIRPEQL TEEFWDFVFL GQGSADEVSK KTGVPVEALK AIREEFEYWY PLDSRNSGKD 

       610        620        630        640        650        660 
LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD RAVEMYGPDP 

       670        680        690        700        710        720 
LRATLALAAE VEQDLDFRDA EARRNAQQLM AIYTLVQRLI QNAENRPAGW IDQWLIAEIS 

       730        740        750        760        770        780 
RILDKAREAY EKVRVRQAAV EVIYNAKAVF DQYLAMVEKP TKLALEAARA WVVAMEPIVP 

       790        800        810        820        830        840 
HIAEELWALL GGSGFVATAP WPRLKAESAA LLAKRYVDML IEDVKNIPAF GPGARRIVIY 

       850        860        870        880        890        900 
VNRNFSWAKA ALNGDVKTVV TAGAPPQVAK RLIDLVKTLG DEVRTLLAST EQFDELETLK 

       910        920        930        940 
SYKAYIERTL GTPIEIYSVD DPSAPDLGGK KRAALPLKPG IYIER 

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References

[1]"Complete sequence of Pyrobaculum islandicum DSM 4184."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 4184 / JCM 9189.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000504 Genomic DNA. Translation: ABL88411.1.
RefSeqYP_930754.1. NC_008701.1.

3D structure databases

ProteinModelPortalA1RTX9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING384616.Pisl_1246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL88411; ABL88411; Pisl_1246.
GeneID4616435.
KEGGpis:Pisl_1246.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAGFYEGVM.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycPISL384616:GC8H-1276-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PYRIL
AccessionPrimary (citable) accession number: A1RTX9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries