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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pyrobaculum islandicum (strain DSM 4184 / JCM 9189)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei641 – 6411ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPISL384616:GC8H-1276-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Pisl_1246
OrganismiPyrobaculum islandicum (strain DSM 4184 / JCM 9189)
Taxonomic identifieri384616 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
ProteomesiUP000002595 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Leucine--tRNA ligasePRO_1000009405Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi384616.Pisl_1246.

Structurei

3D structure databases

ProteinModelPortaliA1RTX9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi638 – 6425"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiYEGVMLV.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1RTX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELSRFFIE IAERWQKRWK ESRVFEPEPT PGVAKYFITA AYPYPNGAVH
60 70 80 90 100
IGHGRTYLIA DVLARFYRHM GRVVLYPMGF HYTGTPILTI AEVIAAGDKT
110 120 130 140 150
VIEEYMEIYG VPEEEIKKMG DPLYLARYFH NRSKMAMERF GLSIDWTREF
160 170 180 190 200
TTIDPEYQRF IQWQFEKLRK KGLIVRGRHP VGWCPRHSMP VGAHDTKDDK
210 220 230 240 250
EPEIGQWTLI YFTDSEGLTF PAATLRPETV PGVTNLWINP DAEYVVAEFD
260 270 280 290 300
GKRVVISRDA AYRLSFQAEV KILREARGRE FVGRSVQNPV TGEWVPIYEA
310 320 330 340 350
WFVDPRVGTG VVMSVPAHAP YDYVALRDLG IEKLIPLIKV EGYGDYPAKE
360 370 380 390 400
VVERMGIKSQ TDPALEEATK EVYSTEYTRG VMREDVTERI GIHLQEPARS
410 420 430 440 450
MLRAVFKMYF AGRPVREARE FIAKWLVEAR LGGVMYDIMN KPVYCRCGTE
460 470 480 490 500
IVVKVLEDQW FINYGDPKWK ETARKLVDEM VIIPGEAKAH FFATIDWLDK
510 520 530 540 550
RACARTRGLG TPLPWSSGWI IESLSDSTIY MAFYTVIKKI RQFGIRPEQL
560 570 580 590 600
TEEFWDFVFL GQGSADEVSK KTGVPVEALK AIREEFEYWY PLDSRNSGKD
610 620 630 640 650
LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD
660 670 680 690 700
RAVEMYGPDP LRATLALAAE VEQDLDFRDA EARRNAQQLM AIYTLVQRLI
710 720 730 740 750
QNAENRPAGW IDQWLIAEIS RILDKAREAY EKVRVRQAAV EVIYNAKAVF
760 770 780 790 800
DQYLAMVEKP TKLALEAARA WVVAMEPIVP HIAEELWALL GGSGFVATAP
810 820 830 840 850
WPRLKAESAA LLAKRYVDML IEDVKNIPAF GPGARRIVIY VNRNFSWAKA
860 870 880 890 900
ALNGDVKTVV TAGAPPQVAK RLIDLVKTLG DEVRTLLAST EQFDELETLK
910 920 930 940
SYKAYIERTL GTPIEIYSVD DPSAPDLGGK KRAALPLKPG IYIER
Length:945
Mass (Da):107,947
Last modified:February 6, 2007 - v1
Checksum:i66351DCA0CEB630D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL88411.1.
RefSeqiYP_930754.1. NC_008701.1.

Genome annotation databases

EnsemblBacteriaiABL88411; ABL88411; Pisl_1246.
GeneIDi4616435.
KEGGipis:Pisl_1246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL88411.1.
RefSeqiYP_930754.1. NC_008701.1.

3D structure databases

ProteinModelPortaliA1RTX9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi384616.Pisl_1246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL88411; ABL88411; Pisl_1246.
GeneIDi4616435.
KEGGipis:Pisl_1246.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiYEGVMLV.

Enzyme and pathway databases

BioCyciPISL384616:GC8H-1276-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Pyrobaculum islandicum DSM 4184."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 4184 / JCM 9189.

Entry informationi

Entry nameiSYL_PYRIL
AccessioniPrimary (citable) accession number: A1RTX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: February 4, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.