A1RT34 (RIFK_PYRIL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Pisl_0940

Organism

Pyrobaculum islandicum (strain DSM 4184 / JCM 9189) [Complete proteome] [HAMAP]

Taxonomic identifier

384616 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Riboflavin kinase HAMAP MF_01285		PRO_0000322104
Regions
	Nucleotide binding	91 – 96	6	CDP By similarity
	Nucleotide binding	190 – 193	4	CDP By similarity
	Region	1 – 81	81	H-T-H motif-like HAMAP MF_01285
	Region	82 – 211	130	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	120	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Binding site	177	1	FMN By similarity
	Binding site	185	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1RT34 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 4CB70DD72EABE67C	FASTA	211	23,601
	10 20 30 40 50 60 MKCIDRRLIG DLIALSTVEG LSVTDAAEKL CLTRQGLYKL LRHLKKEGYV AEGPAIRLTQ 70 80 90 100 110 120 KGKDTLGIVL KDLLRYFNIL SIRLSGRVVS GLGEGAFYMS LEGYRRAIEQ KLGFTPYPGT 130 140 150 160 170 180 LNIKLDPPSV VYKRYLDSLP GILIPGFSNG LRTYGAVKAF RAKLRDIEGA IVMPERTHHP 190 200 210 TDVIEVIAPF KLREILGLRD GDRVEIEIYL E

References

[1]

"Complete sequence of Pyrobaculum islandicum DSM 4184." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 4184 / JCM 9189.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000504 Genomic DNA. Translation: ABL88116.1.
RefSeq	YP_930459.1. NC_008701.1.
3D structure databases
ProteinModelPortal	A1RT34.
ModBase	Search...
Protein-protein interaction databases
STRING	A1RT34.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4616735.
GenomeReviews	Gene locus Pisl_0940 in contig CP000504_GR.
KEGG	pis:Pisl_0940.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	MPERTHH.
ProtClustDB	CLSK631895.
Enzyme and pathway databases
BioCyc	PISL384616:PISL_0940-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRIL

Accession

Primary (citable) accession number: A1RT34

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 6, 2007
Last modified:	November 16, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program