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A1RSJ7 (RTCA_PYRIL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:Pisl_0753
OrganismPyrobaculum islandicum (strain DSM 4184 / JCM 9189) [Complete proteome] [HAMAP]
Taxonomic identifier384616 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_1000012115

Regions

Nucleotide binding286 – 2894ATP By similarity

Sites

Active site3121Tele-AMP-histidine intermediate By similarity
Binding site1011ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1RSJ7 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 4A4891FF6D440D5E

FASTA34837,233
        10         20         30         40         50         60 
MIVKIDGSYG EGGGQILRTS IALSALLGKP VEIINIRAKR TNPGLQPQHL TGVSAAALLT 

        70         80         90        100        110        120 
DADIEGAAKG STRLYFKPKT LKCGTFNIDI GTAGSISLVI QTLAPILLYA PCPTKLVITG 

       130        140        150        160        170        180 
GTDVAWAPPI DYMRYVFVKV LSLFGAKIAI ELVRRGHYPR GGGRVIVYVE PIKKLRAVHY 

       190        200        210        220        230        240 
VEFGRLVKIS GISHAVNLPT HVAERQAKAA KETLAKLGYV AEVEVETRSD GLGPGSGIVL 

       250        260        270        280        290        300 
WAESDIGNVV GGDALGERGK PAEEVGREAA ERLAAVLKTG VSLDPHMADM AVVYMALAEG 

       310        320        330        340 
RSKMSTSEAT LHLQTNMYIV EQFLPVKFKL EKTGARYIIE VDGIGYSR 

« Hide

References

[1]"Complete sequence of Pyrobaculum islandicum DSM 4184."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 4184 / JCM 9189.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000504 Genomic DNA. Translation: ABL87929.1.
RefSeqYP_930272.1. NC_008701.1.

3D structure databases

ProteinModelPortalA1RSJ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING384616.Pisl_0753.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL87929; ABL87929; Pisl_0753.
GeneID4618208.
KEGGpis:Pisl_0753.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
OMARRGHYPK.
ProtClustDBPRK04204.

Enzyme and pathway databases

BioCycPISL384616:GC8H-774-MONOMER.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_PYRIL
AccessionPrimary (citable) accession number: A1RSJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families