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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATPUniRule annotation1
Active sitei312Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi286 – 289ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Pisl_0753
OrganismiPyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Taxonomic identifieri384616 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002595 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000121151 – 348RNA 3'-terminal phosphate cyclaseAdd BLAST348

Interactioni

Protein-protein interaction databases

STRINGi384616.Pisl_0753.

Structurei

3D structure databases

ProteinModelPortaliA1RSJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04125. Archaea.
COG0430. LUCA.
HOGENOMiHOG000015264.
KOiK01974.
OMAiNGCVDEF.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC. 1 hit.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PTHR11096:SF0. PTHR11096:SF0. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1RSJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVKIDGSYG EGGGQILRTS IALSALLGKP VEIINIRAKR TNPGLQPQHL
60 70 80 90 100
TGVSAAALLT DADIEGAAKG STRLYFKPKT LKCGTFNIDI GTAGSISLVI
110 120 130 140 150
QTLAPILLYA PCPTKLVITG GTDVAWAPPI DYMRYVFVKV LSLFGAKIAI
160 170 180 190 200
ELVRRGHYPR GGGRVIVYVE PIKKLRAVHY VEFGRLVKIS GISHAVNLPT
210 220 230 240 250
HVAERQAKAA KETLAKLGYV AEVEVETRSD GLGPGSGIVL WAESDIGNVV
260 270 280 290 300
GGDALGERGK PAEEVGREAA ERLAAVLKTG VSLDPHMADM AVVYMALAEG
310 320 330 340
RSKMSTSEAT LHLQTNMYIV EQFLPVKFKL EKTGARYIIE VDGIGYSR
Length:348
Mass (Da):37,233
Last modified:February 6, 2007 - v1
Checksum:i4A4891FF6D440D5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL87929.1.
RefSeqiWP_011762505.1. NC_008701.1.

Genome annotation databases

EnsemblBacteriaiABL87929; ABL87929; Pisl_0753.
GeneIDi4618208.
KEGGipis:Pisl_0753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL87929.1.
RefSeqiWP_011762505.1. NC_008701.1.

3D structure databases

ProteinModelPortaliA1RSJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi384616.Pisl_0753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL87929; ABL87929; Pisl_0753.
GeneIDi4618208.
KEGGipis:Pisl_0753.

Phylogenomic databases

eggNOGiarCOG04125. Archaea.
COG0430. LUCA.
HOGENOMiHOG000015264.
KOiK01974.
OMAiNGCVDEF.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC. 1 hit.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PTHR11096:SF0. PTHR11096:SF0. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRTCA_PYRIL
AccessioniPrimary (citable) accession number: A1RSJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.