A1RSI5 (ILVD_PYRIL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Pisl_0740

Organism

Pyrobaculum islandicum (strain DSM 4184 / JCM 9189) [Complete proteome] [HAMAP]

Taxonomic identifier

384616 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000001042
Sites
	Metal binding	124	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	196	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1RSI5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 634C96A3A5FBDA83	FASTA	562	59,862
	10 20 30 40 50 60 MVKVRIRSPA WYDGVDNTPH RSYLRAIGFT DEDFTKPLVG VLAAWSELGP CNYHTLELAK 70 80 90 100 110 120 YVKEGIKEAG GVGLIAPTIV VNDGINMGTP GMRYSLISRD LIADTIEAQF NAHGIDAWVG 130 140 150 160 170 180 IGGCDKTQPG IMMAMVRLDL PAVYMYGGTA EAGWLGEREL TIEDTFETVG SYLAGKITLE 190 200 210 220 230 240 ELKRIEELSF PTYGTCQGLF TANTMAILSE ALGLALLGSA SPPATSARRK AYAVASGRAV 250 260 270 280 290 300 LKAAELGITP RKVVTYDAIY NAAVTLFATA GSTNAILHLL AIAYEAGVKF TLDDFDEISR 310 320 330 340 350 360 RVPVIAALRP AGPYAMQDLD RIGGAPRILK KLYKAGLLRP EALTVEGETI GKLLERWQPP 370 380 390 400 410 420 SVPEDGILYS VEKPYKPYSG IRILRGNLAP DGAVMKIGAA DKLKFEGKAK VYNGEAEAFK 430 440 450 460 470 480 AVAAGEIKPG DVVVIRYEGP KGAPGMPEML KVTAAIVGAG LGEAVALVTD GRFSGATRGI 490 500 510 520 530 540 MVGHVAPEAA VGGPIALVEN GDRIAIDGET GRITLQIPQE ELERRRKNWT PPPPKYSGGL 550 560 LAKYAALVQQ ADKGAVTAIP LR

References

[1]

"Complete sequence of Pyrobaculum islandicum DSM 4184." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 4184 / JCM 9189.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000504 Genomic DNA. Translation: ABL87917.1.
RefSeq	YP_930260.1. NC_008701.1.
3D structure databases
ProteinModelPortal	A1RSI5.
ModBase	Search...
Protein-protein interaction databases
STRING	A1RSI5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4616534.
GenomeReviews	Gene locus Pisl_0740 in contig CP000504_GR.
KEGG	pis:Pisl_0740.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04340.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	A1RSI5.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	PISL384616:PISL_0740-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_PYRIL

Accession

Primary (citable) accession number: A1RSI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 16, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program