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Protein

Flap endonuclease 1

Gene

fen

Organism
Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).By similarity

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31Magnesium 1UniRule annotation1
Metal bindingi84Magnesium 1UniRule annotation1
Metal bindingi156Magnesium 1UniRule annotation1
Metal bindingi158Magnesium 1UniRule annotation1
Metal bindingi177Magnesium 2UniRule annotation1
Metal bindingi179Magnesium 2UniRule annotation1
Metal bindingi239Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:Pisl_0681
OrganismiPyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Taxonomic identifieri384616 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000613321 – 346Flap endonuclease 1Add BLAST346

Interactioni

Subunit structurei

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi384616.Pisl_0681.

Structurei

3D structure databases

ProteinModelPortaliA1RSC7.
SMRiA1RSC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 102N-domainAdd BLAST102
Regioni120 – 261I-domainAdd BLAST142

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
KOiK04799.
OMAiGSQDYDS.
OrthoDBiPOG093Z0642.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiView protein in InterPro
IPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiView protein in Pfam
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
PRINTSiPR00853. XPGRADSUPER.
SMARTiView protein in SMART
SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00841. XPG_1. 1 hit.

Sequencei

Sequence statusi: Complete.

A1RSC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVTELGKLI GKDIRREVKL ENLAGKCIAL DAYNSLYQFL ASIRQPDGTP
60 70 80 90 100
LMDRVGRITS HLSGLFYRTI NLMEAGIKPV YVFDGKPPEF KLAEIEERRK
110 120 130 140 150
VKEKAMEEVL KAIKEGRKED VAKYMKRAVF LTSDMVEDAK KLLTYMGVPW
160 170 180 190 200
VQAPSEGEAQ AAHMAKRGHC WAVGSQDYDS LLFGSPRLVR NLAVSPRRKI
210 220 230 240 250
GEEVIELSPE IIELDAVLKS LRLKNREQLI DLAILLGTDY NPDGVPGVGP
260 270 280 290 300
QKALKLVWEF GGLEKMLETV LRGVYFPVDP LEIKKFFLNP PVTDEYSTDI
310 320 330 340
KKPDEQKLID FLVREHDFSE DRVLKAVERL KRAQTKYKIS SLDSFF
Length:346
Mass (Da):39,274
Last modified:February 6, 2007 - v1
Checksum:i8E2C0F76908DDB1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000504 Genomic DNA. Translation: ABL87859.1.
RefSeqiWP_011762435.1. NC_008701.1.

Genome annotation databases

EnsemblBacteriaiABL87859; ABL87859; Pisl_0681.
GeneIDi4616401.
KEGGipis:Pisl_0681.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFEN_PYRIL
AccessioniPrimary (citable) accession number: A1RSC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: March 15, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families