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A1RRG7 (SYE_PYRIL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:Pisl_0371
OrganismPyrobaculum islandicum (strain DSM 4184 / JCM 9189) [Complete proteome] [HAMAP]
Taxonomic identifier384616 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_1000057201

Regions

Motif107 – 11711"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
A1RRG7 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 18DEEF91F7822362

FASTA57065,108
        10         20         30         40         50         60 
MNLEELVLKY ALANAVKYGG KADVKAVMSK IMAEVPELRP KAREVKSMVE AVVARVNSMS 

        70         80         90        100        110        120 
LEEQLRLLRE RWPETLEERR VEQKRPGIEN LAELPNVKGG VVVRFAPNPD FVLHLGSARP 

       130        140        150        160        170        180 
AILNYAYRLK YGGKFILRFE DTDPRTKKPL VTSEINAYET IREDLKWLGI KWDEEYIQSM 

       190        200        210        220        230        240 
RMEIYYEHIK KLLEKGAAYV DLCRPEEWRK LRNAGRACPH REQSPEENLE LWDRMLEGRF 

       250        260        270        280        290        300 
KEGEAVVRIK TDLSHPDPSV RDWVAFRIID TSKTPHPLVG DKYIVWPTYN FAVSIDDHLM 

       310        320        330        340        350        360 
GITHVLRAQE HSVNTIKQSY IFKHFGWEQP VTIHFGRLKI EGASLSKSKL KALGVRYDDI 

       370        380        390        400        410        420 
SLPTLAGLRN RGILPEAIWE LILTVGVKPS DSTIALSNLF ALNRKRIEPI ANRYMYVADP 

       430        440        450        460        470        480 
IKLVFKSDRE LLAKIPLHPS FRERGERIYK FGPGTIELFV PRQDIKPGAV VRLMELANVE 

       490        500        510        520        530        540 
IIAVENGVAH GRLHSVGIDE ARKVGAPIIQ WVYEPIEIHV VKPVAVGKKV EEIGLGESAL 

       550        560        570 
EKVETGAYVQ FMRYGFLKKV GPSAFVYVHD 

« Hide

References

[1]"Complete sequence of Pyrobaculum islandicum DSM 4184."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 4184 / JCM 9189.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000504 Genomic DNA. Translation: ABL87549.1.
RefSeqYP_929892.1. NC_008701.1.

3D structure databases

ProteinModelPortalA1RRG7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING384616.Pisl_0371.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL87549; ABL87549; Pisl_0371.
GeneID4616813.
KEGGpis:Pisl_0371.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAAFRIIDT.

Enzyme and pathway databases

BioCycPISL384616:GC8H-377-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_PYRIL
AccessionPrimary (citable) accession number: A1RRG7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries