A1RQQ2 (PURL_PYRIL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Pisl_0101

Organism

Pyrobaculum islandicum (strain DSM 4184 / JCM 9189) [Complete proteome] [HAMAP]

Taxonomic identifier

384616 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050339
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1RQQ2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 989DE1EF35F6F44F	FASTA	693	74,726
	10 20 30 40 50 60 MGLTQRELEI IRERLGREPT AAELAFFTSH WSEHCSYKST KMWLRELPGS APWVVRGRGT 70 80 90 100 110 120 DAPLVEVAHG LYVSFKIESH NHPSAVDPYN GAATGVGGII RDILTVGATP IALLVNLHFG 130 140 150 160 170 180 PPEDPHAKWI FSNVIKGISD YGNRVGVPVV GGETWFDEDF TYTPIVLATC VGVVEAEAVP 190 200 210 220 230 240 KGYVEPGDYI VVAGLGADRS GLGGSAFASK TLEGGEDLGA IQVADPLMGK KLIDVVKEVS 250 260 270 280 290 300 RCVKFIKDLG GGGLATALAE LSHWFSLGVE FHLDKLHVRD ASALPEELLI SETQERLIFV 310 320 330 340 350 360 ISPRDLPCLE EALRKYEVPY SIPGRFVKSD RVLVMWKGVK IVDIPISLAD GAPEILWPQE 370 380 390 400 410 420 PYQPSDIPEI PEPPIDKAID IVLSSPNVAK KESIYMRFDF DVGVRTAVKP GEGDAAVLKL 430 440 450 460 470 480 YQRGQLGLVV KGDANPRYTF LDPRLGAANA FVKAYRNVAV VGGVPLAAVD SINVGSPERP 490 500 510 520 530 540 QVYWRFVQAV QGLKEAATEL EVPIVGGKVS LYNEYMGRPI KPTVAVVVLG KIDDVSKANR 550 560 570 580 590 600 ALWHDGDKIY IWGVTKGEVG GSEYLKRVHG ILAGRPPSID YAAEKEIVAV IQSWLGKISG 610 620 630 640 650 660 ATDVGLGGLI TALAKMAINS GIGGEIDVCK APAETTRLDY LLFSESNGRF IATGAEGPGV 670 680 690 AIGEAYGEEF VVKCGGTLIY KRGVEELKRL MSL

References

[1]

"Complete sequence of Pyrobaculum islandicum DSM 4184." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 4184 / JCM 9189.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000504 Genomic DNA. Translation: ABL87284.1.
RefSeq	YP_929627.1. NC_008701.1.
3D structure databases
ProteinModelPortal	A1RQQ2.
ModBase	Search...
Protein-protein interaction databases
STRING	A1RQQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4616954.
GenomeReviews	Gene locus Pisl_0101 in contig CP000504_GR.
KEGG	pis:Pisl_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	PISL384616:PISL_0101-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRIL

Accession

Primary (citable) accession number: A1RQQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program