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A1RLX8 (GLGB_SHESW) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Sputw3181_2856
OrganismShewanella sp. (strain W3-18-1) [Complete proteome] [HAMAP]
Taxonomic identifier351745 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length745 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7457451,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000045000

Sites

Active site4161Nucleophile By similarity
Active site4691Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A1RLX8 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: E9BD3D7EAC4EA9C6

FASTA74584,750
        10         20         30         40         50         60 
MMTQANAYFY NGADVALLNG QYTDVFSLLG MHTSEDGKSL IVRCFLRNAL KVDVISIKDG 

        70         80         90        100        110        120 
RRVAGLEKVN DAGLFAGTMG RRVKPFLYLL RIQYPLSQIE IVDPYQFGPL LNADDLYLFG 

       130        140        150        160        170        180 
EGSAERAYEF LGANWRDVEG IEGVHFCVWA PNAKRVSVVG DFNHWDDTRH VMRQHMANGL 

       190        200        210        220        230        240 
WEIFLPNVAE GAHYKFDLVH PNGERHAKSD PMATQMECAP HNASIVPKKS KHSWKDTLWM 

       250        260        270        280        290        300 
NKRAVTAWHK APMAIYEVHL GSWRRKGDNG EQYLDYQDLI EQLIPYVKAQ SFTHIELMPI 

       310        320        330        340        350        360 
SEYPFDGSWG YQPVGLYAPT HRFGDANGLK AFVDACHQAD IGVVLDWVAA HFPKDPHGLV 

       370        380        390        400        410        420 
RFDGTCLYEH EDPRKGTHPD WDTLIYNYGR GEVRSFLLSN ACYWLREFHF DGLRIDAVSS 

       430        440        450        460        470        480 
MLYLDYSREP GQWLPNAYGG RENLEAISFL QMLNQRLYQA FPGICMIAEE STAFAGVTKP 

       490        500        510        520        530        540 
TDHHGLGFGF KWNMGWMNDS LSYLSRDPIY RQYHHHQLTF SLMYAYTEQF MLSVSHDEVV 

       550        560        570        580        590        600 
HGKGSLLHKI PGDDWQKFAT LRAYYGFMWG HPGKKLLFMG SEFAQRDEWD HNHSLDWHLL 

       610        620        630        640        650        660 
AFEPHQGVQR WLKDLNQLYQ GMSALSVLDY QPAGFRWLDC DNGSASIFTF VRYGLAGDAP 

       670        680        690        700        710        720 
LVFVINMTPS VHHGFRIGLP QAGDFCEYLN SDSYLYGGSN QGNAGLVVAQ NQSWQGMESS 

       730        740 
ALITVPPLSC LVLGPVANQV EAKRR 

« Hide

References

[1]"Complete sequence of Shewanella sp. W3-18-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., Tiedje J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: W3-18-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000503 Genomic DNA. Translation: ABM25673.1.
RefSeqYP_964227.1. NC_008750.1.

3D structure databases

ProteinModelPortalA1RLX8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351745.Sputw3181_2856.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM25673; ABM25673; Sputw3181_2856.
GeneID4658183.
KEGGshw:Sputw3181_2856.
PATRIC23599517. VBISheSp103602_2983.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSSP351745:GCOY-2953-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SHESW
AccessionPrimary (citable) accession number: A1RLX8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries