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A1RGN2

- DNAJ_SHESW

UniProt

A1RGN2 - DNAJ_SHESW

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Protein
Chaperone protein DnaJ
Gene
dnaJ, Sputw3181_0977
Organism
Shewanella sp. (strain W3-18-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity.UniRule annotation

Cofactori

Binds 2 zinc ions per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi145 – 1451Zinc 1 By similarity
Metal bindingi148 – 1481Zinc 1 By similarity
Metal bindingi162 – 1621Zinc 2 By similarity
Metal bindingi165 – 1651Zinc 2 By similarity
Metal bindingi184 – 1841Zinc 2 By similarity
Metal bindingi187 – 1871Zinc 2 By similarity
Metal bindingi198 – 1981Zinc 1 By similarity
Metal bindingi201 – 2011Zinc 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri132 – 21079CR-typeUniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSSP351745:GCOY-997-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJ
Gene namesi
Name:dnaJ
Ordered Locus Names:Sputw3181_0977
OrganismiShewanella sp. (strain W3-18-1)
Taxonomic identifieri351745 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000002597: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Chaperone protein DnaJUniRule annotation
PRO_1000085300Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi351745.Sputw3181_0977.

Structurei

3D structure databases

ProteinModelPortaliA1RGN2.
SMRiA1RGN2. Positions 2-75, 132-210.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7066J
Add
BLAST
Repeati145 – 1528CXXCXGXG motifUniRule annotation
Repeati162 – 1698CXXCXGXG motifUniRule annotation
Repeati184 – 1918CXXCXGXG motifUniRule annotation
Repeati198 – 2058CXXCXGXG motifUniRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi77 – 11539Gly-richUniRule annotation
Add
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.
Contains 1 J domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
HOGENOMiHOG000226717.
KOiK03686.
OMAiGHTDPNQ.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1RGN2-1 [UniParc]FASTAAdd to Basket

« Hide

MSKRDYYEVL GVGRDASERE IKKAYKRLAM KFHPDRNPGD KAAEASFKEA    50
KEAYEILTDT DKKAAYDQFG HAGVDPNRGG GYGGGQGDFG DIFGDVFGDI 100
FGGGRRGGQR QAARGSDLRY NLELSLEEAV KGLTKELRIP TLATCDLCDG 150
SGAKKGTSAS TCTTCHGQGQ VQMRQGFFTV QQPCPTCHGR GKIIKDPCSK 200
CHGDGRVEKS KTLSVKIPAG VDTGDRIRLA GEGEAGEFGA PPGDLYVQVS 250
VREHAIFVRD GNNLYCEVPI SFSKAALGGE IEVPTLDGKV SLKIPAETQT 300
GRMFRLRGKG VKSVRSHAVG DLLCKVVMET PVNLNDRQKE LLREFEATLT 350
GESKKHSPKA EGFFDGVKKF FQDLNS 376
Length:376
Mass (Da):40,737
Last modified:February 6, 2007 - v1
Checksum:iCC2FD660033D35DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000503 Genomic DNA. Translation: ABM23827.1.
RefSeqiYP_962381.1. NC_008750.1.

Genome annotation databases

EnsemblBacteriaiABM23827; ABM23827; Sputw3181_0977.
GeneIDi4659522.
KEGGishw:Sputw3181_0977.
PATRICi23595423. VBISheSp103602_1015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000503 Genomic DNA. Translation: ABM23827.1 .
RefSeqi YP_962381.1. NC_008750.1.

3D structure databases

ProteinModelPortali A1RGN2.
SMRi A1RGN2. Positions 2-75, 132-210.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 351745.Sputw3181_0977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM23827 ; ABM23827 ; Sputw3181_0977 .
GeneIDi 4659522.
KEGGi shw:Sputw3181_0977.
PATRICi 23595423. VBISheSp103602_1015.

Phylogenomic databases

eggNOGi COG0484.
HOGENOMi HOG000226717.
KOi K03686.
OMAi GHTDPNQ.
OrthoDBi EOG6BPDKP.

Enzyme and pathway databases

BioCyci SSP351745:GCOY-997-MONOMER.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: W3-18-1.

Entry informationi

Entry nameiDNAJ_SHESW
AccessioniPrimary (citable) accession number: A1RGN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi