Periplasmic nitrate reductase precursor - Shewanella sp. (strain W3-18-1)

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A1RG51 (A1RG51_SHESW) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 51. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase HAMAP-Rule MF_01630
EC=1.7.99.4 HAMAP-Rule MF_01630

Gene names

Name:	napA HAMAP-Rule MF_01630
Ordered Locus Names:	Sputw3181_0795

Organism

Shewanella sp. (strain W3-18-1) [Complete proteome] [HAMAP] EMBL ABM23646.1

Taxonomic identifier

351745 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630 SAAS SAAS006656
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 1 molybdenum ion per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Subunit structure	Interacts with NapB By similarity. HAMAP-Rule MF_01630 SAAS SAAS019546
Subcellular location	Periplasm By similarity HAMAP-Rule MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven By similarity. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. HAMAP-Rule MF_01630

Ontologies

Keywords
Biological process	Electron transport HAMAP-Rule MF_01630 SAAS SAAS006656 Nitrate assimilation HAMAP-Rule MF_01630 SAAS SAAS006656 Transport
Cellular component	Periplasm HAMAP-Rule MF_01630
Domain	Signal HAMAP-Rule MF_01630 EMBL ABM23646.1
Ligand	4Fe-4S HAMAP-Rule MF_01630 SAAS SAAS006656 Iron Iron-sulfur Metal-binding Molybdenum HAMAP-Rule MF_01630 SAAS SAAS010051
Molecular function	Oxidoreductase SAAS SAAS006656 HAMAP-Rule MF_01630
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 33

Potential EMBL ABM23646.1

Signal peptide

1 – 32

Tat-type signal By similarity HAMAP-Rule MF_01630

Chain

34 – 826

793

Potential EMBL ABM23646.1

PRO_5000203760

Sites

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Sequences

Sequence

Length

Mass (Da)

Tools

A1RG51 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: EA75ECD77474B12E
Show »

FASTA

826

92,335

        10         20         30         40         50         60 
MSISRREFLK ANAALAAATA VGVTLPVKMV EAAESDNIKW DKAPCRFCGV GCSVLVGTKA 

        70         80         90        100        110        120 
GKVVATKGDP ESPVNRGLNC IKGYFLSKIM YGKDRLTTPL LRMKDGKYHK EGEFTPVSWD 

       130        140        150        160        170        180 
VALDTMAAKW KHSIATKGPT SVGMFGSGQW TIWEGYAASK LHKAGFLTNN IDPNARHCMA 

       190        200        210        220        230        240 
SAVVGFMRTF GIDEPMGCYD DLEAADHFVL WGANMAEMHP ILWARLSDRR LSSPSCRVHV 

       250        260        270        280        290        300 
LSTFENRSFD LADNPMVFHP QSDLVILNYI ANYIIQNKAV NTDFVTKHTQ FALGVDDIGY 

       310        320        330        340        350        360 
GLRPDHPLEK KAKNPGNGKS TPISFDEYAK FVSTYTLEYA AKMSGVEPEK LETLAKAYAD 

       370        380        390        400        410        420 
PKVKVMSLWT MGINQHVRGV WANNMLYNLH LLTGKIATPG NSPFSLTGQP SACGTAREVG 

       430        440        450        460        470        480 
TFAHRLPADM VVDNDKHRAI TEKMWQVPEG TIPPKPGYHA VLQSRMLKDG KLNCYWTMCT 

       490        500        510        520        530        540 
NNMQAGPNIN EEMYPGFRNP ENFIVVSDPY PTVTAMAADL ILPTAMWVEK EGAYGNAERR 

       550        560        570        580        590        600 
THMWHQQVKA PEGAKSDLWQ LMEFAKRFNV SEVWPAELIA KQPEYADKTL FEVLFANGVI 

       610        620        630        640        650        660 
NKFPTTDCKA ELNDESQHFG FYVQKGIFEE YAAFGRGHAH DLADFDRYHE TRGLRWPVVN 

       670        680        690        700        710        720 
GKETLRRFVE GSDPYVKAGE GFKFYGKPDG KAVIFALPYE PAAEEPNSEY DLWMSTGRVL 

       730        740        750        760        770        780 
EHWHTGSMTA RVPELYRAYP DAQIFMHPED AKARGLQRGD EVVVASPRGE VKTRVETKGR 

       790        800        810        820 
NKPPRGVVFM PFFDARQLVN KLILDATDPL SKETDFKKCP VKVMKA

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References

[1]

"Complete sequence of Shewanella sp. W3-18-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.

Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: W3-18-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000503 Genomic DNA. Translation: ABM23646.1.
RefSeq	YP_962200.1. NC_008750.1.
3D structure databases
ProteinModelPortal	A1RG51.
SMR	A1RG51. Positions 38-825.
ModBase	Search...
Protein-protein interaction databases
STRING	351745.Sputw3181_0795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM23646; ABM23646; Sputw3181_0795.
GeneID	4659879.
KEGG	shw:Sputw3181_0795.
PATRIC	23595035. VBISheSp103602_0822.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031441.
KO	K02567.
OMA	NMLYNIH.
ProtClustDB	PRK13532.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1RG51_SHESW

Accession

Primary (citable) accession number: A1RG51

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information