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A1RG51

- A1RG51_SHESW

UniProt

A1RG51 - A1RG51_SHESW

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella sp. (strain W3-18-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotationSAAS annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi52 – 521Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Electron transportUniRule annotationSAAS annotation, Nitrate assimilationUniRule annotationSAAS annotation, Transport

    Keywords - Ligandi

    4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, MolybdenumUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciSSP351745:GCOY-815-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Sputw3181_0795Imported
    OrganismiShewanella sp. (strain W3-18-1)Imported
    Taxonomic identifieri351745 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002597: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    PeriplasmUniRule annotation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333 PotentialImportedAdd
    BLAST
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini34 – 826793 PotentialImportedPRO_5000203760Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi351745.Sputw3181_0795.

    Structurei

    3D structure databases

    ProteinModelPortaliA1RG51.
    SMRiA1RG51. Positions 38-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 94574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation
    Contains 4Fe-4S Mo/W bis-MGD-type domain.SAAS annotation

    Keywords - Domaini

    SignalUniRule annotationImported

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiRRLSHKD.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1RG51-1 [UniParc]FASTAAdd to Basket

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    MSISRREFLK ANAALAAATA VGVTLPVKMV EAAESDNIKW DKAPCRFCGV    50
    GCSVLVGTKA GKVVATKGDP ESPVNRGLNC IKGYFLSKIM YGKDRLTTPL 100
    LRMKDGKYHK EGEFTPVSWD VALDTMAAKW KHSIATKGPT SVGMFGSGQW 150
    TIWEGYAASK LHKAGFLTNN IDPNARHCMA SAVVGFMRTF GIDEPMGCYD 200
    DLEAADHFVL WGANMAEMHP ILWARLSDRR LSSPSCRVHV LSTFENRSFD 250
    LADNPMVFHP QSDLVILNYI ANYIIQNKAV NTDFVTKHTQ FALGVDDIGY 300
    GLRPDHPLEK KAKNPGNGKS TPISFDEYAK FVSTYTLEYA AKMSGVEPEK 350
    LETLAKAYAD PKVKVMSLWT MGINQHVRGV WANNMLYNLH LLTGKIATPG 400
    NSPFSLTGQP SACGTAREVG TFAHRLPADM VVDNDKHRAI TEKMWQVPEG 450
    TIPPKPGYHA VLQSRMLKDG KLNCYWTMCT NNMQAGPNIN EEMYPGFRNP 500
    ENFIVVSDPY PTVTAMAADL ILPTAMWVEK EGAYGNAERR THMWHQQVKA 550
    PEGAKSDLWQ LMEFAKRFNV SEVWPAELIA KQPEYADKTL FEVLFANGVI 600
    NKFPTTDCKA ELNDESQHFG FYVQKGIFEE YAAFGRGHAH DLADFDRYHE 650
    TRGLRWPVVN GKETLRRFVE GSDPYVKAGE GFKFYGKPDG KAVIFALPYE 700
    PAAEEPNSEY DLWMSTGRVL EHWHTGSMTA RVPELYRAYP DAQIFMHPED 750
    AKARGLQRGD EVVVASPRGE VKTRVETKGR NKPPRGVVFM PFFDARQLVN 800
    KLILDATDPL SKETDFKKCP VKVMKA 826
    Length:826
    Mass (Da):92,335
    Last modified:February 6, 2007 - v1
    Checksum:iEA75ECD77474B12E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000503 Genomic DNA. Translation: ABM23646.1.
    RefSeqiYP_962200.1. NC_008750.1.

    Genome annotation databases

    EnsemblBacteriaiABM23646; ABM23646; Sputw3181_0795.
    GeneIDi4659879.
    KEGGishw:Sputw3181_0795.
    PATRICi23595035. VBISheSp103602_0822.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000503 Genomic DNA. Translation: ABM23646.1 .
    RefSeqi YP_962200.1. NC_008750.1.

    3D structure databases

    ProteinModelPortali A1RG51.
    SMRi A1RG51. Positions 38-825.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 351745.Sputw3181_0795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM23646 ; ABM23646 ; Sputw3181_0795 .
    GeneIDi 4659879.
    KEGGi shw:Sputw3181_0795.
    PATRICi 23595035. VBISheSp103602_0822.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi RRLSHKD.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci SSP351745:GCOY-815-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: W3-18-1Imported.

    Entry informationi

    Entry nameiA1RG51_SHESW
    AccessioniPrimary (citable) accession number: A1RG51
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 6, 2007
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3