A1RFQ1 (TPMT_SHESW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Sputw3181_0645

Organism

Shewanella sp. (strain W3-18-1) [Complete proteome] [HAMAP]

Taxonomic identifier

351745 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047226
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1RFQ1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: E3CE5EC2454B649E	FASTA	218	25,059
	10 20 30 40 50 60 MEPGFWHEKW YKQQIGFHQQ DINPFLVQYW QRLALPAGAK VFVPLCGKSL DMCFLAEQGH 70 80 90 100 110 120 QVIGCELNEL AVQQFFSDNQ LEMTQTVEGE HQHYQTEQVS LYQGDIFTLP KRITQDVTAF 130 140 150 160 170 180 YDRAALIAWP EEMRAQYAKQ LANLLPSGSL GLLVTLDYPQ ETLNGPPFAV SPNWIEAHLT 190 200 210 DDFEIQALAC QDVLADNPRF VKKEVPWLNE AAYLLKRK

References

[1]

"Complete sequence of Shewanella sp. W3-18-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Tiedje J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: W3-18-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000503 Genomic DNA. Translation: ABM23496.1.
RefSeq	YP_962050.1. NC_008750.1.
3D structure databases
ProteinModelPortal	A1RFQ1.
ModBase	Search...
Protein-protein interaction databases
STRING	A1RFQ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4660418.
GenomeReviews	Gene locus Sputw3181_0645 in contig CP000503_GR.
KEGG	shw:Sputw3181_0645.
NMPDR	fig|351745.7.peg.605.
PATRIC	23594713. VBISheSp103602_0665.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG444929.
OMA	QGDIFTL.
ProtClustDB	PRK13255.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR022474. Thiopur_S-MeTfrase_Se/Te_detox. IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
TIGRFAMs	TIGR03840. TMPT_Se_Te. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_SHESW

Accession

Primary (citable) accession number: A1RFQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program