ID   FTHS_SHESW              Reviewed;         570 AA.
AC   A1RFN3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   27-MAR-2024, entry version 81.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Sputw3181_0627;
OS   Shewanella sp. (strain W3-18-1).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=351745;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=W3-18-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Tiedje J., Richardson P.;
RT   "Complete sequence of Shewanella sp. W3-18-1.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000503; ABM23478.1; -; Genomic_DNA.
DR   RefSeq; WP_011788008.1; NC_008750.1.
DR   AlphaFoldDB; A1RFN3; -.
DR   SMR; A1RFN3; -.
DR   KEGG; shw:Sputw3181_0627; -.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002597; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..570
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000300541"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   570 AA;  60137 MW;  C4A5431C9C60FE1C CRC64;
     MLTDMEISSR ASLTNVAELG AELGLLPEEM LLFGSTKAKV DLSVQQRLAG QSRGKLIIVT
     AVTPTPHGEG KTVTSIGLTQ SLKAIGKKAC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
     ELNLHLTGDI HAVSSAHNLG AAAIAARLFH ESRLGKVEFE AQSGQAFLDI DPAQIRWHRV
     LDHNDRCLRQ VQVGLGENNG PVYESGFDIT AASELMAILA LSANLADMRA RIGRLVLALN
     RQGQVISAED LGVAGAMTAI MVDAIKPTLM QTLNGAPCLI HAGPFANIAH GNSSIIADDI
     ALKLVDYVIT EGGFGSDMGF EKFCNIKVRQ SGQVPSAAVL VTTLKALKAN SGIESDTDIN
     TPDEIRLEAG FANLHWHINN VARYGIPVVV AINRFATDTE AELQWLINAV NQTAAFGCEV
     SDAFSQGEAG AIELAYTVVR AADTQSQFRL LYPDDASLEA KLSTLAEVGY GAAGISLSDE
     AKQQVREFTS LGYAYLPVCM AKTPLSISHD PMQKGVPKDF IVPIRALVLN AGAGFVTALV
     GNVMTMPGLG IKPGYLKIDI DEAGEIIGLG
//