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A1RAI8 (TRMB_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:AAur_3557
OrganismArthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]
Taxonomic identifier290340 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288118

Regions

Region280 – 2834Substrate binding By similarity

Sites

Active site1601 By similarity
Binding site851S-adenosyl-L-methionine By similarity
Binding site1101S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1601S-adenosyl-L-methionine By similarity
Binding site1641Substrate By similarity
Binding site1961Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1RAI8 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 7665727B93B13679

FASTA30133,299
        10         20         30         40         50         60 
MSETPDSPRP VTPGSQASFG TYGGRPVSFV RRGTRLQGRR QAAWEEHAER WAIQVPRHVA 

        70         80         90        100        110        120 
NTSVHPDYTF DAEAVFGRKA PLIVEIGSGL GDAVVHAAEQ NPDKDFLAVE VYTPGLANTI 

       130        140        150        160        170        180 
IKINSRGLTN VRVVEANAPE VLESMLPEGS VSELWVFFPD PWHKARHHKR RLIQPAFASV 

       190        200        210        220        230        240 
AAKALVKGGY WRIATDWSNY AVHVREVLTG STEFENMHEG ERSGEESPLT QVWQSGVESV 

       250        260        270        280        290        300 
VGGAPVREGR APVSTEHTGP NEGVDEEGGW APRFDGRIRT SFENKAHEAG RMIFDLTYRK 


R 

« Hide

References

[1]"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1."
Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.
PLoS Genet. 2:2094-2106(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000474 Genomic DNA. Translation: ABM06497.1.
RefSeqYP_949250.1. NC_008711.1.

3D structure databases

ProteinModelPortalA1RAI8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290340.AAur_3557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM06497; ABM06497; AAur_3557.
GeneID4639067.
KEGGaau:AAur_3557.
PATRIC20982652. VBIArtAur67810_3629.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAANTSVHP.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK960282.

Enzyme and pathway databases

BioCycAAUR290340:GI59-3556-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ARTAT
AccessionPrimary (citable) accession number: A1RAI8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways