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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Paenarthrobacter aurescens (strain TC1)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Sitei104Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Binding sitei265Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processGlycolysisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:AAur_2976Imported
OrganismiPaenarthrobacter aurescens (strain TC1)Imported
Taxonomic identifieri290340 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePaenarthrobacter
Proteomesi
  • UP000000637 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi290340.AAur_2976.

Structurei

3D structure databases

ProteinModelPortaliA1R8X2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 295PFKInterPro annotationAdd BLAST294

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni271 – 274Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK21071.
OMAiRDGWRGP.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiView protein in InterPro
IPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
PfamiView protein in Pfam
PF00365. PFK. 1 hit.
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiView protein in PROSITE
PS00433. PHOSPHOFRUCTOKINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A1R8X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGILTSGG DCPGLNAVIR GSVLKGIAIH GQEFVGFRDG WRGVVEGDVI
60 70 80 90 100
DIPRTMVRGI AKQGGTILGT SRTNPFENGG GPDVIKAHME RLGIDAIIAI
110 120 130 140 150
GGEGTLAAAK RLTDAGLKIV GVPKTVDNDL DATDYTFGFD TAVQIATEAI
160 170 180 190 200
DRLRTTGESH HRCMIAEVMG RHVGWIALHA GMAAGAHAIL IPEQKASIEQ
210 220 230 240 250
ITEWVKEAHD RGRAPLVVVA EGFVPDHMES PHSERGLDTF GRPRLGGIAD
260 270 280 290 300
QLAPEIEART GIETRATILG HIQRGGVPTA YDRVLATRLG MAAIDSVVEG
310 320 330 340
RWGTMVALKG TDISHVGFEE ALGKLKTVPQ HRYDEASVLF G
Length:341
Mass (Da):36,246
Last modified:February 6, 2007 - v1
Checksum:i9EFBE3D10FA5471A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM09472.1.
RefSeqiWP_011775619.1. NC_008711.1.

Genome annotation databases

EnsemblBacteriaiABM09472; ABM09472; AAur_2976.
GeneIDi29622223.
KEGGiaau:AAur_2976.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA1R8X2_PAEAT
AccessioniPrimary (citable) accession number: A1R8X2
Entry historyiIntegrated into UniProtKB/TrEMBL: February 6, 2007
Last sequence update: February 6, 2007
Last modified: June 7, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported