A1R8P2 (A1R8P2_ARTAT) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Name:	treZ EMBL ABM08308.1
Ordered Locus Names:	AAur_2895

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP] EMBL ABM08308.1

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 EMBL ABM08308.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	259	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	296	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	392	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A1R8P2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 62BDA2DB1D49AE23	FASTA	590	65,038
	10 20 30 40 50 60 MLEHAAGKNR YDVWAPNAGS VRLLADDREY AMEKHHDGAA SGWWRAPADV VAEETDGVRY 70 80 90 100 110 120 GYVLDGEGPF PDPRSRRQPD GVHQLSATFD PSVHEWKDSE WKGRNLKGSV IYELHLGTFT 130 140 150 160 170 180 PEGTLDAAAG KLDYLVDLGV DFIELLPVNG FNGVHNWGYD GVLWYAVHEP YGGPAAYQRF 190 200 210 220 230 240 VDAAHAAGLG VIQDVVYNHL GPSGNYLPKF GPYLKSGEGN TWGDSVNLDG PGSDDVRRYI 250 260 270 280 290 300 LDNAAMWLRD YHVDGLRLDA VHAFRDERAV HILEEFGALG DQIEAETGIP RTLIAESDLN 310 320 330 340 350 360 NPRLIYPSQD NGYGLEGQWS DDFHHAVHVN LSGETTGYYS DFDSLAVLAK VLEHGFLHDG 370 380 390 400 410 420 SYSSFRGRHH GRPIRRDLAH PSALVVCLQN HDQIGNRAIG DRLTATLSYG QLAIGAVLTM 430 440 450 460 470 480 TSPFTPMLFM GEEFGASTPW QFFTSHPEPE LGKATAEGRI KEFERMGWDP AVVPDPQDPE 490 500 510 520 530 540 TFQRSKLKWD EIGDGDHARL LQLYKDLAQL RRNTPELVEG GFGETSVEFD DDEHWLQFTR 550 560 570 580 590 GTVRVVCNFG GDAFETPLSG SVLLATDGEA ALEAGTLTLP GASAAVVRVQ

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM08308.1.
RefSeq	YP_948604.1. NC_008711.1.
3D structure databases
ProteinModelPortal	A1R8P2.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R8P2.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4640575.
GenomeReviews	Gene locus AAur_2895 in contig CP000474_GR.
KEGG	aau:AAur_2895.
PATRIC	20981270. VBIArtAur67810_2943.
TIGR	AAur_2895.
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG367595.
OMA	PGKRNWG.
ProtClustDB	CLSK849609.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1R8P2_ARTAT

Accession

Primary (citable) accession number: A1R8P2

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)