Reviewed, UniProtKB/Swiss-Prot A1R8N9 (FTHS_ARTAT)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	AAur_2892

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000293031
Regions
	Nucleotide binding	70 – 77	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R8N9-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 994851454A18EF7C	FASTA	562	59,201
	10 20 30 40 50 60 MSENKVLSDL EIAHNATMLP VEAIAERAGI NVDALELYGP YKAKINTAKL VLPRGKAPGK 70 80 90 100 110 120 VVLVSAMSPT PAGEGKSTTT VGLADSLARA GHKVMIALRE PSLGPILGMK GGATGGGYSQ 130 140 150 160 170 180 VLPMDDINLH FTGDFHAITS ANNALMALVD NHIFQGNQLG IDPRRMTFKR VLDMNDRALR 190 200 210 220 230 240 EVVIGLGGPM QGVPRQDGFD ITVASEIMAV FCLATDLDDL RERLGRITFG YTYDRTPVTV 250 260 270 280 290 300 SDLGVEGALT MLLKDAIKPN LVQTIAGTPA LVHGGPFANI AHGCNSLIAT STAMQLADIV 310 320 330 340 350 360 VTEAGFGADL GAEKYMDIKA RIAEVAPSAV VVVATIRALK MQGGVPKEKL NEPNVEAVAA 370 380 390 400 410 420 GVENLKRHVG NVAKFGISPV VSINKFGSDT PEELEWLLAW CAAEGVEAAV ADVWGRGGGG 430 440 450 460 470 480 DGGDELAAKV AAALEAPSNF HHLYPLELGV EDKIRTIVQE IYGADGVEFS VPALKRLAEI 490 500 510 520 530 540 EKNGWSGLPV CMAKTQYSFT DDASRLGAPK GFRVHVRELI PKTGAGFIVA LTGAVMTMPG 550 560 LPKEPAAMRM DVDADGNPTG LF

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM10024.1.
RefSeq	YP_948601.1.
3D structure databases
SMR	A1R8N9. Positions 9-560.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R8N9.
Genome annotation databases
GeneID	4639848.
GenomeReviews	Gene locus AAur_2892 in contig CP000474_GR.
KEGG	aau:AAur_2892.
TIGR	AAur_2892.
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HBG677721.
OMA	GMKGGAT.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_ARTAT

Accession

Primary (citable) accession number: A1R8N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 6, 2007
Last modified:	January 19, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)