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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Arthrobacter aurescens (strain TC1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081IronUniRule annotation
Metal bindingi112 – 1121IronUniRule annotation
Binding sitei183 – 1831SubstrateUniRule annotation
Binding sitei196 – 1961Substrate; via amide nitrogenUniRule annotation
Binding sitei200 – 2001SubstrateUniRule annotation
Binding sitei301 – 3011SubstrateUniRule annotation
Metal bindingi329 – 3291IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciAAUR290340:GI59-2882-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:AAur_2883
OrganismiArthrobacter aurescens (strain TC1)
Taxonomic identifieri290340 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePaenarthrobacter
Proteomesi
  • UP000000637 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303257Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290340.AAur_2883.

Structurei

3D structure databases

ProteinModelPortaliA1R8N0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni149 – 1535Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiDHVHEVS.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.

Sequencei

Sequence statusi: Complete.

A1R8N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGIESSCDE TGVGIVRGTT LLTNTVSSSM DEHVRFGGVI PEIASRAHLD
60 70 80 90 100
AFVPTLQESL QEAGVTLEDI DAIAVTSGPG LAGALMVGVC AAKALAVATG
110 120 130 140 150
KPLYAINHLV AHVGVGLLDG NRVSEGKHDA VAAAGLGAGK LPENLGALLV
160 170 180 190 200
SGGHTEILRI RSITDDVELL GSTIDDAAGE AYDKVARILG LGYPGGPAID
210 220 230 240 250
KLAHQGNPKS IRFPRGLTQP KYMGTAEEKG PHRYDWSFSG LKTAVARCVE
260 270 280 290 300
QFEARGEEVP VADIAAAFQE AVVDVISSKA VLACKEHGIT DVLLGGGVAA
310 320 330 340 350
NSRLRELTGQ RCASAGITLH VPPLGLCTDN GAMVAALGAQ LIMAGISPSG
360
VSFAPDSSMP VTTVSVPA
Length:368
Mass (Da):37,580
Last modified:February 6, 2007 - v1
Checksum:i2B3DA8282E043407
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM08610.1.

Genome annotation databases

EnsemblBacteriaiABM08610; ABM08610; AAur_2883.
KEGGiaau:AAur_2883.
PATRICi20981246. VBIArtAur67810_2931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM08610.1.

3D structure databases

ProteinModelPortaliA1R8N0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290340.AAur_2883.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM08610; ABM08610; AAur_2883.
KEGGiaau:AAur_2883.
PATRICi20981246. VBIArtAur67810_2931.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiDHVHEVS.
OrthoDBiEOG6K402S.

Enzyme and pathway databases

BioCyciAAUR290340:GI59-2882-MONOMER.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TC1.

Entry informationi

Entry nameiTSAD_ARTAT
AccessioniPrimary (citable) accession number: A1R8N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 6, 2007
Last modified: June 8, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.