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A1R7K6 (SYE_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:AAur_2494
OrganismArthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]
Taxonomic identifier290340 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000330952

Regions

Motif29 – 3911"HIGH" region HAMAP-Rule MF_00022
Motif273 – 2775"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2761ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1R7K6 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 166719215BB883C6

FASTA50655,751
        10         20         30         40         50         60 
MTTASASNAA SSAIPAVNAE TPVRVRFCPS PTGTPHVGLI RTALFNWAYA RHTGGKLIFR 

        70         80         90        100        110        120 
IEDTDSARDS EESYHQLLDA LKWLGINWDE GVEVGGPHEP YRQSQRGDIY QDVIAKLKAG 

       130        140        150        160        170        180 
GHVYESYSTP DEIEARHKAA GRDPKLGYDG FDRHLTDEQL AQFKAEGREA VLRLRMPDED 

       190        200        210        220        230        240 
LTFNDLVRGE ITFKAGSVPD FAVVRANGAP LYTLVNPVDD ALMGITHVLR GEDLLSSTPR 

       250        260        270        280        290        300 
QIALYRALYA IGVAEYMPEF GHLPYVMGQG NKKLSKRDPE SSLFLHRERG FIPEGLLNYL 

       310        320        330        340        350        360 
SLLGWSLSAD EDIFTVEQLV ANFDIHDVLG NPARFDIKKA EAINGTHVRM LAAEDFKERL 

       370        380        390        400        410        420 
VPYLRAAGFV GEILTPRQEE ILAEAAPLVQ ERITLLGEAP EMLAFLFKAD DAIDVADDAR 

       430        440        450        460        470        480 
KGLPQNLEEV LDAALAALEP IGEWTAENIQ TALKQALVED LGIKPRAAFG PVRTAISGRR 

       490        500 
ISPPLFESMV ILGKDSSLAR VRAFRG 

« Hide

References

[1]"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1."
Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.
PLoS Genet. 2:2094-2106(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000474 Genomic DNA. Translation: ABM07629.1.
RefSeqYP_948218.1. NC_008711.1.

3D structure databases

ProteinModelPortalA1R7K6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290340.AAur_2494.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM07629; ABM07629; AAur_2494.
GeneID4640794.
KEGGaau:AAur_2494.
PATRIC20980422. VBIArtAur67810_2526.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAAFRCFCT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycAAUR290340:GI59-2493-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_ARTAT
AccessionPrimary (citable) accession number: A1R7K6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries