Reviewed, UniProtKB/Swiss-Prot A1R7G3 (TRMD_ARTAT)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	AAur_2449

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006447
Regions
	Region	137 – 142	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	113	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R7G3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 5A151BA98DD0BCD2	FASTA	266	29,401
	10 20 30 40 50 60 MRIDVVSIFP EYLAPLELSL IGKARQDGLL ELNVHDLRTF TTDKHRTVDD TPYGGGAGMV 70 80 90 100 110 120 MKPEPWAQAL ESVAAARENS KPVLIVPSPA GEKFNQALAY ELAEEEQLVF ACGRYEGIDE 130 140 150 160 170 180 RVIDWAQDHF RVRPVSLGDY VLNGGEVAVL AMVEAIGRLL PGVVGNPESL VEESHSDGLL 190 200 210 220 230 240 EYPVYTKPSA WRDHDVPPIL LSGNHGKIAQ WRRHEQFRRT AERRPDLMEG LDAGVLSRAD 250 260 RQALADLGYD VVDGRLRAKP GGDTEN

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM06607.1.
RefSeq	YP_948175.1.
3D structure databases
SMR	A1R7G3. Positions 1-250.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R7G3.
Genome annotation databases
GeneID	4638249.
GenomeReviews	Gene locus AAur_2449 in contig CP000474_GR.
KEGG	aau:AAur_2449.
TIGR	AAur_2449.
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ARTAT

Accession

Primary (citable) accession number: A1R7G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)