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A1R7G3 (TRMD_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:AAur_2449
OrganismArthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]
Taxonomic identifier290340 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

Subunit structure

Homodimer By similarity. HAMAP MF_00605

Subcellular location

Cytoplasm Potential HAMAP MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N1-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
PRO_1000006447

Regions

Region137 – 1426S-adenosyl-L-methionine binding By similarity

Sites

Binding site1131S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A1R7G3 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 5A151BA98DD0BCD2

FASTA26629,401
        10         20         30         40         50         60 
MRIDVVSIFP EYLAPLELSL IGKARQDGLL ELNVHDLRTF TTDKHRTVDD TPYGGGAGMV 

        70         80         90        100        110        120 
MKPEPWAQAL ESVAAARENS KPVLIVPSPA GEKFNQALAY ELAEEEQLVF ACGRYEGIDE 

       130        140        150        160        170        180 
RVIDWAQDHF RVRPVSLGDY VLNGGEVAVL AMVEAIGRLL PGVVGNPESL VEESHSDGLL 

       190        200        210        220        230        240 
EYPVYTKPSA WRDHDVPPIL LSGNHGKIAQ WRRHEQFRRT AERRPDLMEG LDAGVLSRAD 

       250        260 
RQALADLGYD VVDGRLRAKP GGDTEN

« Hide

References

[1]"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1."
Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.
PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000474 Genomic DNA. Translation: ABM06607.1.
RefSeqYP_948175.1. NC_008711.1.

3D structure databases

ProteinModelPortalA1R7G3.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1R7G3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4638249.
GenomeReviewsGene locus AAur_2449 in contig CP000474_GR.
KEGGaau:AAur_2449.
PATRIC20980330. VBIArtAur67810_2482.
TIGRAAur_2449.

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHBG285805.
OMAVCGRFEG.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycAAUR290340:AAUR_2449-MONOMER.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. TrmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_ARTAT
AccessionPrimary (citable) accession number: A1R7G3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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