Reviewed, UniProtKB/Swiss-Prot A1R735 (SYT_ARTAT)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	AAur_2309

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000020337
Regions
	Region	260 – 566	307	Catalytic HAMAP MF_00184
Sites
	Metal binding	365	1	Zinc; catalytic By similarity
	Metal binding	416	1	Zinc; catalytic By similarity
	Metal binding	543	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R735-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 4F6B8B0F6ED5C894	FASTA	669	75,089
	10 20 30 40 50 60 MSDAQQITLI VDGEETKVTE GTTGAELFFE RRDVVVARVN GVLKDLDQVL TEGADVEGVT 70 80 90 100 110 120 IDSPDGLNVL RHSTAHVMAQ AVQQLRPEAK LGIGPYITDG FYFDFDVADP FTPEDLRTLE 130 140 150 160 170 180 KMMQKIINQN QKFVRRVVTE EEAREAMANE PYKLELLGKK NDASDAAEGV NVEVGAGDIT 190 200 210 220 230 240 IYDNVDRKSG ESIWCDLCRG PHLQNTKIIS NAFALTRSSA AYWLGNQNNQ QLQRIYGTAW 250 260 270 280 290 300 PTKEALKAYQ ERIAEAERRD HRKLGVELDL FSFPDELGSG LPVFHPKGGI IRKAMEDYSR 310 320 330 340 350 360 QRHVDAGYEF VYTPHITKGH LYEVSGHLDW YKDGMFPAMQ VDAEFNEDGS VRKPAQDYYL 370 380 390 400 410 420 KPMNCPMHNL IFRSRGRSYR ELPLRLFEFG SVYRYEKSGV VHGLTRVRGM TQDDAHIYCT 430 440 450 460 470 480 REQMKDELTT TLNFVLGLLK DYGLDDFYLE LSTKNEEKFV GDDAAWEEAT RTLSEVAEAS 490 500 510 520 530 540 GLHLVPDPGG AAFYGPKISV QAKDALGRTW QMSTIQLDFN LPERFELEYQ AADGTRQRPV 550 560 570 580 590 600 MIHRALFGSV ERFMGVLTEH YAGAFPAWLA PVQVVGIPVA ETFNDYMFDV VGQLKAAGIR 610 620 630 640 650 660 AEVDISSDRF PKKIRTASKD KIPFVLIAGG EDAEAGAVSF RFRDGSQDNG VPVAEAVRRI VDAVKNRES

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000474 Genomic DNA. Translation: ABM09654.1.
RefSeq	YP_948047.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1R735.
Genome annotation databases
GeneID	4637449.
GenomeReviews	Gene locus AAur_2309 in contig CP000474_GR.
KEGG	aau:AAur_2309.
TIGR	AAur_2309.
Phylogenomic databases
OMA	NTKIAIG.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ARTAT

Accession

Primary (citable) accession number: A1R735

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)