A1R709 (SYA_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AAur_2281

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence ABM07701.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347492
Sites
	Metal binding	577	1	Zinc Potential
	Metal binding	581	1	Zinc Potential
	Metal binding	680	1	Zinc Potential
	Metal binding	684	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A1R709 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 94F591DEFFE5B4A3	FASTA	892	95,822
	10 20 30 40 50 60 MKSQEITKRW VDFFVNKGHT AVPSASLVSS DPSLLFTVAG MVPFIPYLTA REEPPYSRAT 70 80 90 100 110 120 SVQKCIRTGD IEEVGKTARH GTFFQMCGNF SFGDYFKEDA IKFAFELLTT SVDDGGYGLP 130 140 150 160 170 180 AERLWVTVYE EDDEAKELWL KNTGIPAERI QRMGKADNYW STGQPGPAGP CSEIYYDRGP 190 200 210 220 230 240 AYGIEGGPLA DETRYIEIWN LVFMQYQIEN VRSKVDFDIV GELPQKNIDT GLGMERLAMI 250 260 270 280 290 300 LQGVENMYET DQVRPVIDKA AELSGREYTS AESPEDPHHT DDVRMRVVGD HIRSALMLIA 310 320 330 340 350 360 DGVAPSNEGR GYVLRRLIRR AVRAMRLLGV EKACLPDLLP ASRDAMKGVY PVVETDFARI 370 380 390 400 410 420 SRIAYAEEKA FLRTIASGTA RLEDAVAESK AAGVPLSGAD AFALHDTYGF PIDLTLEMAE 430 440 450 460 470 480 EAGLQVDEAG FRALMLEQRQ RAQADAKGKK GGHADLSAYQ ELLGKGETVF TGYDELEGEA 490 500 510 520 530 540 KVRGIVSGGR AVAHASTGDE IELVLNETPF YAEAGGQSAD TGLITGDGFV VEVLDVQRPI 550 560 570 580 590 600 KGLSVHKAIV REGEIGSDAL VRAAVDRERR HAAEQAHTGT HIVHAALHQI LGPEATQRGS 610 620 630 640 650 660 YNKAGYLRFD FAWGEGLSTA TRSEIEEVSN IAIRNNFRVD TKVMGLAEAK ALGAMALFGE 670 680 690 700 710 720 NYGSEVRVVE IDGAWSRELC GGTHVSNTSL IGSLSLLGEQ SVGSGNRRVE AFVGLDAFRH 730 740 750 760 770 780 LAAERALVSE LTELLKVPSG QLADRISSTL NKLKATEKEL DRLRKEQLAA AAANLVGTAK 790 800 810 820 830 840 DAAGVRVVAH DAGQVGGADD LRNLALDLRN RLGSEASTVA VAGVSNDRPV ILIATNEAAR 850 860 870 880 890 EAGVKAGALV RLAAGILGGG GGGKDDVAQG GGTDAGKVSE ALTAVVDAIA KR

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM07701.1. Different initiation.
RefSeq	YP_948021.1. NC_008711.1.
3D structure databases
ProteinModelPortal	A1R709.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4639186.
GenomeReviews	Gene locus AAur_2281 in contig CP000474_GR.
KEGG	aau:AAur_2281.
PATRIC	20979984. VBIArtAur67810_2316.
TIGR	AAur_2281.
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	AAUR290340:AAUR_2281-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ARTAT

Accession

Primary (citable) accession number: A1R709

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program